Rabbit anti-Human IL9 Monoclonal Antibody for IF/ICC and FC (intra) - P15248

Core Product Specifications and Parameters

Biological Background: Interleukin-9 Function and Localization

• Interleukin-9 (IL-9) is a multifunctional cytokine, also known as Cytokine P40 and T-cell growth factor P40, secreted primarily by T-helper 2 lymphocytes, mast cells, and NKT cells.
• IL-9 plays important roles in the immune response against parasites and affects intestinal epithelial permeability and adaptive immunity. Related references: PMID:29742432
• It induces the differentiation of specific T-cell subsets, such as IL-17 producing helper T-cells (TH17), and promotes the proliferation and differentiation of mast cells.
• IL-9 signals through a heterodimeric receptor composed of the IL-9 receptor (IL9R) and the common gamma chain (IL2RG), activating JAK1/JAK3 kinases and downstream STAT1, STAT3, and STAT5 transcriptional programs.
• Subcellularly, IL-9 is a secreted protein that functions as an extracellular cytokine.
• Post-translational modifications include glycosylation and a pyrrolidone carboxylic acid residue; it belongs to the cytokine/growth factor family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-144 of human IL-9, covering the mature secreted form; consider using this antibody for detection of the processed cytokine in biological samples.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to allow access to the secreted or intracellular pool of IL-9.
• For intracellular flow cytometry (FC), use standard intracellular staining protocols with appropriate permeabilization buffers and include unstimulated controls to assess background.
• As IL-9 is secreted, stimulation of cells with PMA/ionomycin or antigen in the presence of a secretion inhibitor (e.g., Brefeldin A) may enhance detection.
• Validate antibody specificity using IL-9 knockout or over-expression cell lines, or by pre-incubation with recombinant IL-9 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, bulk ancillary reagents, and rare target sourcing solutions. This anti-IL-9 antibody supports cytokine immunology research and diagnostic assay development. Benefit from our streamlined procurement for monoclonal and polyclonal antibodies, recombinant proteins, and technical consulting tailored to your project needs.