Rabbit anti-Human IL8 Monoclonal Antibody for Intracellular Flow Cytometry - P10145

Core Product Specifications and Parameters

Biological Background: IL8 Function and Localization

• Interleukin-8 (IL-8), encoded by the CXCL8 gene, is a C-X-C motif chemokine that plays a central role in immune and inflammatory responses.
• It acts as a potent chemoattractant for neutrophils, basophils, and T-cells, directing these immune cells to sites of infection or tissue damage. Related references: PMID:18692776, PMID:7636208
• IL-8 is critical for neutrophil activation, enhancing their antimicrobial functions. Related references: PMID:2145175, PMID:9623510
• It signals through the G protein-coupled receptors CXCR1 and CXCR2, triggering downstream PI3K and MAPK pathways upon ligand binding. Related references: PMID:1840701, PMID:1891716, PMID:11971003, PMID:8662698
• The protein is secreted and functions as a soluble cytokine.
• IL-8 contains conserved disulfide bonds and undergoes citrullination, post-translational modifications that may influence its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen encompasses the mature IL-8 sequence (aa 28–99), expected to target the secreted, biologically active form. For intracellular flow cytometry, ensure permeabilization and stimulation protocols are optimized for cytokine detection.
• The antibody is validated for human IL-8 with intracellular FC; cross-reactivity with other species or isoforms has not been demonstrated.
• Consider testing other immunoassays (e.g., ELISA, Western blot) after thorough in-house validation with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research labs with a streamlined pathway to high-quality IVD raw materials, including validated monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For IL-8, a key inflammatory biomarker, our supply network supports cytokine detection assay development with reliable antibody reagents. Reach out to explore custom procurement solutions.