Rabbit anti-Human IL8 Monoclonal Antibody for Flow Cytometry - P10145

Core Product Specifications and Parameters

Biological Background: IL8 Function and Localization

• Interleukin-8 (IL8), encoded by the CXCL8 gene, is a C-X-C motif chemokine that serves as a central mediator of the inflammatory response, attracting neutrophils, basophils, and T-cells to sites of infection or injury (PubMed:18692776, PubMed:7636208). Related references: PMID:18692776 PMID:7636208
• IL8 directly activates neutrophils, enhancing their ability to clear pathogens, a critical step in innate immunity (PubMed:2145175, PubMed:9623510). Related references: PMID:2145175 PMID:9623510
• These functions are elicited through binding to the G protein-coupled receptors CXCR1 and CXCR2, which are predominantly expressed on neutrophils, monocytes, and endothelial cells (PubMed:1840701, PubMed:1891716). Related references: PMID:1840701 PMID:1891716
• Receptor activation triggers dissociation of the G protein heterotrimer, leading to downstream signaling via PI3K and MAPK pathways, which further amplify the inflammatory cascade (PubMed:11971003, PubMed:8662698). Related references: PMID:11971003 PMID:8662698
• The mature IL8 protein is approximately 11 kDa and is secreted from cells, with conserved disulfide bonds critical for its structural integrity.
• Post-translational modifications of IL8, including citrullination, may modulate its activity and stability in the extracellular environment.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, ensure optimal fixation and permeabilization protocols to allow the conjugated antibody access to cytoplasmic IL8.
• Consider titrating the antibody to determine the ideal working dilution for your specific cell type and stimulation conditions.
• As this antibody is specific for human IL8, verify reactivity if applied to non-human samples; cross-reactivity with other species is not indicated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a seamless supply of premium IVD raw materials, including expertly validated antibodies like this anti-IL8 clone for inflammation studies. We provide comprehensive sourcing solutions—from optimized monoclonal and polyclonal antibodies to bulk ancillary reagents and rare-target procurement—to accelerate your project from concept to clinical application.