Rabbit anti-Human IL8 Monoclonal Antibody for Flow Cytometry (Intracellular) - P10145

Core Product Specifications and Parameters

Biological Background: IL8 Function and Localization

• Interleukin-8 (IL8), also known as CXCL8, is a C-X-C motif chemokine that plays a key role in inflammatory responses.
• Functions as a chemotactic factor, attracting neutrophils, basophils, and T-cells to sites of infection or injury to clear pathogens and protect the host. Related references: PMID:18692776 PMID:7636208
• Activates neutrophils, promoting their migration and degranulation. Related references: PMID:2145175 PMID:9623510
• Exerts its effects by binding to G protein-coupled receptors CXCR1 and CXCR2 on target cells, leading to downstream signaling through PI3K and MAPK pathways. Related references: PMID:1840701 PMID:1891716 PMID:11971003 PMID:8662698
• IL8 is a secreted protein encoded by the CXCL8 gene in humans (Gene ID: 3576).
• The mature protein has a molecular weight of approximately 11 kDa and contains conserved disulfide bonds essential for its structure.
• Post-translational modifications include citrullination, which may regulate its activity.
• It is classified as an inflammatory cytokine and is a member of the intercrine alpha (CXC) chemokine family.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment covering amino acids 28-99 of human IL8. For intracellular flow cytometry, permeabilization and fixation are required; consider optimizing permeabilization buffers and antibody dilution.
• Validate the antibody in relevant human sample types (e.g., stimulated PBMCs) and include appropriate isotype controls.
• Protect the fluorochrome conjugate from light and avoid repeated freeze-thaw cycles to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner, CamelBio provides validated antibodies, bulk ancillary reagents, and customized raw material solutions for diagnostic and research applications. This anti-IL8 antibody is available for inflammatory disease research, and we offer expert support for assay development and protein expression services.