Rabbit anti-Human IL23R Monoclonal Antibody for Flow Cytometry - Q5VWK5

Core Product Specifications and Parameters

Biological Background: IL23R Function and Localization

• Interleukin-23 receptor (IL23R) is a transmembrane protein that, together with IL12RB1, forms the heterodimeric receptor for interleukin-23 (IL-23).
• Binding of IL-23 to the receptor complex activates the JAK-STAT signaling pathway, primarily stimulating T-cells, NK cells, and certain macrophage/myeloid cells.
• IL23R plays a key role in innate and adaptive immunity, participating in the acute response to infection in peripheral tissues and contributing to autoimmune inflammatory diseases.
• It is implicated in tumorigenesis, suggesting a role in cancer immunology.
• The receptor is located on the cell membrane and is expressed by various immune cells including monocytes, Th1, Th0, NK cells, and dendritic cells; isoform 1 is specifically expressed in NK cells.
• IL23R undergoes alternative splicing and glycosylation; it is a phosphoprotein with signal peptide and transmembrane domains.
• The canonical isoform has a predicted molecular weight of ~72 kDa, with additional bands at ~41 kDa and ~43 kDa likely representing isoforms or cleavage products.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 124-353 of human IL23R (extracellular domain). This design is well-suited for flow cytometry applications targeting cell surface receptors.
• For FC, titrate the antibody to determine the optimal staining concentration using relevant cell lines or primary cells such as activated T cells or NK cells.
• Include appropriate isotype controls and Fc receptor blocking to ensure specificity.
• The conjugate is bright and photostable; use standard red laser excitation (633–647 nm) and protect from prolonged light exposure.
• Avoid freeze-thaw cycles; store at 2–8°C and minimize exposure to light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including this anti-IL23R monoclonal antibody. Our supply capabilities cover validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Accelerate your immunology and oncology assays from concept to clinic with CamelBio as your trusted partner.