Rabbit anti-Human IL18R1/IL-18 R alpha Monoclonal Antibody for FC - Q13478

Core Product Specifications and Parameters

Biological Background: IL18R1 Function and Localization

• IL18R1 (CD218a, IL-1Rrp) is the ligand-binding subunit of the interleukin-18 receptor complex, specifically recognizing the pro-inflammatory cytokine IL18 (PubMed:14528293, PubMed:25261253, PubMed:25500532).
  Related references:
  PMID:14528293 PMID:25261253 PMID:25500532

• Upon IL18 binding, IL18R1 pairs with IL18RAP to initiate signal transduction, culminating in NF-kB activation and IFNG synthesis in T-helper 1 (Th1) cells (PubMed:10653850).
  Related references:
  PMID:10653850

• The receptor is predominantly localized to the cell membrane, consistent with its role in extracellular cytokine sensing and transmembrane signaling.

• Expression is highest in leukocytes, spleen, and lung, with lower levels in liver, intestine, prostate, thymus, placenta, and heart; IL18R1 is specifically coexpressed with IL18 in Th1 cells (PubMed:10653850, PubMed:10925275, PubMed:11046021).
  Related references:
  PMID:10653850 PMID:10925275 PMID:11046021

• IL18R1 contains three extracellular immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic Toll/IL-1 receptor (TIR) domain, characteristic of the IL-1 receptor family.

• Post-translational modifications include N-glycosylation and disulfide bonds, contributing to structural stability and ligand interaction.

• Keywords from UniProt highlight its involvement in inflammatory response, as a receptor with signal peptide, immunoglobulin domain, and transmembrane regions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–329), supporting the antibody's use for surface staining in flow cytometry applications.
• Being an conjugate, this antibody emits in the far-red spectrum, compatible with common multicolor flow cytometry panels; however, verify compatibility with your instrument configuration.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and sample preparation protocol.
• Include appropriate isotype controls and Fc receptor blocking to minimize non-specific binding, especially when analyzing leukocyte populations.
• Although primarily targeted for surface IL18R1, intracellular staining may be possible after optimizing fixation and permeabilization conditions; but the receptor is mostly surface-localized.
• Validate antibody specificity using IL18R1-overexpressing or knockdown cell lines to confirm signal dependence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes through every stage from concept to clinic. This -conjugated anti-human IL18R1 monoclonal antibody targets a key receptor in inflammatory signaling, ideal for immunology-based assays. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare target raw materials to streamline your development pipeline.