Rabbit anti-Human IL18R1 Monoclonal Antibody for Flow Cytometry - Q13478

Core Product Specifications and Parameters

Biological Background: IL18R1 Function and Localization

• IL18R1, also known as CD218a, IL-1Rrp, or IL-18R-alpha, is a member of the interleukin-1 receptor family and functions as the ligand-binding component of the IL18 receptor complex.
• The receptor specifically binds the pro-inflammatory cytokine IL18, but not IL1A or IL1B, initiating downstream signaling pathways. Related references: PMID:14528293, PMID:25261253, PMID:25500532
• IL18R1-mediated signaling promotes interferon-gamma (IFNG) production in T-helper 1 (Th1) cells, playing a critical role in Th1-type immune responses. Related references: PMID:10653850
• The protein contributes to IL18-induced cytokine production, either independently of SLC12A3 or as part of a complex with SLC12A3 (By similarity).
• IL18R1 is a transmembrane glycoprotein with an extracellular immunoglobulin domain, a single transmembrane helix, and a cytoplasmic TIR domain, featuring disulfide bonds and N-linked glycosylation.
• Subcellularly, IL18R1 localizes to the plasma membrane, consistent with its role as a cell surface receptor.
• The receptor is highly expressed in leukocytes, spleen, and lung, with lower levels in liver, small intestine, colon, prostate, thymus, placenta, and heart; it is specifically coexpressed with IL18R1 in Th1 cells. Related references: PMID:10653850, PMID:10925275, PMID:11046021
• The protein is annotated with keywords including immunoglobulin domain, inflammatory response, and glycoprotein, highlighting its structural and functional roles in immune signaling.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 22-329), making this conjugate well-suited for surface staining in live-cell flow cytometry.
• For FC experiments, consider titrating the antibody to determine the optimal concentration for your specific cell type and instrument setup.
• Include appropriate isotype controls and viability dyes to discriminate specific binding and ensure accurate gating.
• IL18R1 is expressed on Th1 cells; validating staining with known positive (e.g., stimulated human PBMCs) and negative (e.g., Th2 cells) populations is recommended.
• Although validated for flow cytometry, cross-application may require individual optimization and verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies. For targets like IL18R1 in immunological and inflammatory disease research, we offer bulk reagent supply and support for assay development from concept to clinic.