Rabbit anti-Human IL-5 Monoclonal Antibody for Flow Cytometry - P05113

Core Product Specifications and Parameters

Biological Background: IL-5 Function and Localization

• Interleukin-5 (IL-5), also known as eosinophil differentiation factor (EDF), T-cell replacing factor (TRF), and B-cell differentiation factor I, is a homodimeric cytokine primarily produced by T-lymphocytes and NK cells.
• It plays a central role in eosinophil biology, promoting their survival, differentiation, and chemotaxis (PubMed:2653458, PubMed:9010276).
  Related references:
  PMID:2653458
  PMID:9010276
• IL-5 also acts on activated and resting B-cells to stimulate immunoglobulin production, growth, and differentiation.
• The biological effects of IL-5 are mediated through a cell-surface receptor composed of an IL-5-specific alpha subunit (IL5RA) and a common beta subunit (CSF2RB) shared with IL-3 and GM-CSF receptors (PubMed:1495999, PubMed:22528658).
  Related references:
  PMID:1495999
  PMID:22528658
• Upon receptor binding, IL-5 activates intracellular kinases including LYN, SYK, and JAK2, propagating signals through the RAS-MAPK and JAK-STAT5 pathways (PubMed:7613138).
  Related references:
  PMID:7613138
• IL-5 is a secreted glycoprotein, and its presence has been detected in peripheral blood mononuclear cells.
• The protein contains disulfide bonds that are essential for its dimeric structure and biological activity. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature form of human IL-5 (amino acids 20-134), ensuring recognition of the secreted, biologically active protein.
• This conjugate is designed for direct intracellular flow cytometry; fixation and permeabilization steps are required for optimal detection.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and stimulation conditions.
• As IL-5 is secreted, include a protein transport inhibitor (e.g., brefeldin A) during cell stimulation to retain the cytokine intracellularly for staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this Rabbit anti-Human IL-5 mAb as part of a broad portfolio of IVD raw materials tailored for immunology and cytokine research. With capabilities spanning validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we support diagnostic developers from concept to clinic. Our sourcing expertise ensures reliable access to specialty reagents, including conjugated antibodies for multi-parameter analysis.