Rabbit anti-Human IL-4 Monoclonal Antibody for Intracellular Flow Cytometry - P05112

Core Product Specifications and Parameters

Biological Background: IL-4 Function and Localization

• IL-4 is a cytokine that regulates antibody production, hematopoiesis, inflammation, and effector T-cell development. Related references: PMID:1993171, PMID:3016727
• It induces expression of class II MHC molecules on resting B-cells and enhances secretion and cell surface expression of IgE and IgG1. Related references: PMID:1993171
• IL-4 regulates expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Related references: PMID:2521231
• Positively regulates IL31RA expression in macrophages and stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4.
• Upon binding to IL4, IL4R receptor dimerizes with IL2RG (type 1 complex) or IL13RA1 (type 2 complex), activating JAK3/JAK1 and STAT6. Related references: PMID:10219247, PMID:11526337, PMID:18243101, PMID:7721895
• Plays a critical role in higher brain functions such as memory and learning (By similarity).
• Secreted by mast cells, T-cells, eosinophils, and basophils; subcellular location is secreted.
• Keywords: Alternative splicing, B-cell activation, cytokine, glycoprotein.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, stimulate cells and use a protein transport inhibitor (e.g., brefeldin A or monensin) to block secretion, as IL-4 is a secreted cytokine. Consider titrating the antibody to determine the optimal staining concentration.
• Validate staining specificity with appropriate isotype controls and cytokine-specific blocking or unstimulated samples.
• Ensure instrument settings are optimized for the fluorophore (excitation 491 nm, emission 518 nm).
• As a recombinant monoclonal antibody, expect high lot-to-lot consistency and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive sourcing solution for IVD raw materials, from concept to clinic. This anti-human IL-4 antibody supports immunology and inflammation research, and we can assist with validated antibody pairs, bulk ancillary reagents, and rare target material procurement. We aim to be your reliable partner in accelerating immunoassay development.