Rabbit Anti-Human IL-2 Monoclonal Antibody for Intracellular Flow Cytometry - P60568

Core Product Specifications and Parameters

Biological Background: IL-2 Function and Localization

• Interleukin-2 (IL-2), also known as T-cell growth factor (TCGF), is a cytokine primarily produced by activated CD4-positive helper T-cells and, to a lesser extent, by activated CD8-positive T-cells and natural killer (NK) cells. Related references: PMID:6438535
• IL-2 binds to either high-affinity trimeric IL-2R (CD25/CD122/CD132) or low-affinity dimeric IL-2R (CD122/CD132) receptor complexes, inducing downstream JAK/STAT, PI3K, and MAPK signaling cascades. Related references: PMID:16293754 PMID:16477002 PMID:7973659 PMID:8580378 PMID:25142963
• As a T-cell growth factor, IL-2 promotes the proliferation of activated T-cells and enhances NK-cell cytolytic activity. Related references: PMID:6608729
• IL-2 stimulates strong proliferation of activated B-cells and subsequent immunoglobulin production. Related references: PMID:6438535
• It is critical for regulatory T-cell (Treg) survival and function, thereby maintaining immune tolerance, and it also regulates differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, and memory CD8-positive T-cells.
• The protein is secreted and undergoes post-translational modifications including glycosylation and disulfide bond formation, consistent with its keywords "Glycoprotein" and "Disulfide bond".
• IL-2 is classified under keywords such as "Adaptive immunity", "Proto-oncogene", and "Pharmaceutical", reflecting its role in immune regulation and therapeutic targeting.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 21–153, corresponding to the mature secreted IL-2, indicating the antibody likely recognizes the native form.
• For intracellular flow cytometry, optimize fixation/permeabilization (e.g., 4% PFA with saponin or methanol) and titrate the antibody to determine the optimal staining concentration.
• Protect the fluorophore-conjugated antibody from light during handling and incubation to preserve signal intensity.
• Include appropriate controls (isotype control, unstimulated cells, etc.) to validate staining specificity.
• The predicted molecular weight of IL-2 is ~18 kDa; if exploring additional applications, adjust gel percentage and transfer conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and labs with high-quality antibodies, antigens, and ancillary reagents. This IL-2 monoclonal antibody supports intracellular flow cytometry for immunology research, including cytokine signaling and immune cell profiling. With offerings like validated antibody pairs, bulk reagents, and rare target sourcing, CamelBio streamlines your procurement from concept to clinic.