Rabbit anti-Human IL-1β mAb for Intracellular Flow Cytometry - P01584

Core Product Specifications and Parameters

Biological Background: Interleukin-1 beta Function and Localization

• IL-1β (Interleukin-1 beta, also known as Catabolin) is a potent pro-inflammatory cytokine and a member of the interleukin-1 family. It is encoded by the IL1B gene in humans (Gene ID: 3553).
• IL-1β acts as a key endogenous pyrogen, inducing prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell stimulation, and fibroblast proliferation and collagen production. Related references: PMID:3920526
• It promotes Th17 differentiation and synergizes with IL-12 to induce IFN-γ synthesis from Th1 cells. Related references: PMID:10653850
• IL-1β contributes to angiogenesis by inducing VEGF production in synergy with TNF and IL-6. Related references: PMID:12794819
• Following inflammasome activation and pyroptosis, mature IL-1β is released through gasdermin-D (GSDMD) pores, playing a critical role in transducing inflammation downstream of cell death. Related references: PMID:33377178 PMID:33883744
• During Streptococcus pyogenes skin infection, IL-1β acts as a sensor: it is cleaved and activated by the bacterial protease SpeB, triggering an inflammatory response that limits bacterial growth. Related references: PMID:28331908
• Subcellularly, IL-1β is found in the cytoplasm, cytosol, lysosomes, and is secreted extracellularly, including via exosomes.
• It is primarily expressed in activated monocytes and macrophages at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to mature IL-1β (aa 117-269), implying the antibody may detect both pro- and cleaved forms. For intracellular flow cytometry, ensure thorough fixation and permeabilization.
• Optimize permeabilization conditions (e.g., saponin or methanol) to preserve epitope accessibility while allowing antibody entry. Include a viability dye to exclude dead cells if needed.
• Validate the antibody in your specific cellular context by stimulating with known inflammasome activators (e.g., LPS + ATP) and comparing to unstimulated controls.
• Perform a titration experiment to determine the optimal antibody concentration, balancing specific signal against background in permeabilized cells.
• This antibody is specific for human IL-1β; cross-reactivity with mouse or rat IL-1β is not expected based on the immunogen sequence, but should be verified if used in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this -conjugated anti-IL-1β monoclonal antibody, to support diagnostic development and immunology research. Our one-stop sourcing covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target procurement, enabling a seamless transition from concept to clinic. Whether you require off-the-shelf reagents or custom solutions for inflammation targets, CamelBio serves as your reliable partner.