Rabbit Anti-Human IL-17RB Monoclonal Antibody for Flow Cytometry - Q9NRM6

Core Product Specifications and Parameters

Biological Background: IL-17RB Function and Localization

• Interleukin-17 receptor B (IL-17RB) is also known as cytokine receptor-like 4 (CRL4), IL-17 receptor homolog 1 (IL-17Rh1), and IL-17B receptor.
• It functions as a receptor for the pro-inflammatory cytokines IL-17B and IL-17E, mediating downstream signaling pathways.
• May play a role in controlling the growth and/or differentiation of hematopoietic cells, linking it to immune and inflammatory processes.
• The protein is encoded by the IL17RB gene (GeneID: 55540), and alternative splicing generates multiple isoforms (keywords: Alternative splicing).
• IL-17RB is localized primarily on the cell membrane but can also be secreted, consistent with its receptor and soluble forms.
• Post-translational modifications include glycosylation (keyword: Glycoprotein), which may affect its function and stability.
• Tissue specificity: Expressed in several endocrine tissues including fetal and adult liver, kidney, pancreas, testis, colon, brain, and small intestine; not detected in peripheral blood leukocytes or lymphoid organs.
• The molecular weight of the unmodified precursor is approximately 55.9 kDa (UniProt), but observed protein weight varies (31 kDa and 55 kDa) due to isoforms and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 18-289) of human IL-17RB, which is ideal for recognizing both membrane-bound and secreted forms of the receptor.
• This ABflo 488-conjugated monoclonal antibody is supplied for flow cytometry; consider titrating the antibody to optimize staining for your specific cell type.
• For functional studies, note that IL-17RB can exist as a soluble receptor, so cell fixation and permeabilization protocols may need adjustment to avoid loss of signal.
• Validate specificity using positive (IL-17RB-expressing) and negative control cells; blocking with recombinant IL-17B/E may confirm receptor binding specificity.

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