Rabbit anti-Human IL-10RA/CD210 mAb for FC - Q13651

Core Product Specifications and Parameters

Biological Background: IL-10RA/CD210 Function and Localization

• Interleukin-10 receptor subunit alpha (IL-10RA), also designated CD210, IL-10R1, or CDw210a, is a transmembrane receptor belonging to the type II cytokine receptor family.
• It serves as the high-affinity ligand-binding subunit for the anti-inflammatory cytokine IL-10, playing a critical role in limiting excessive immune responses and tissue damage.
• Upon IL-10 binding, IL-10RA undergoes a conformational change that enables recruitment and binding of the IL-10RB co-receptor, forming a functional heterotetrameric signaling complex. Related references: PMID:16982608
• This complex activates the receptor-associated tyrosine kinases JAK1 and TYK2, which phosphorylate specific IL-10RA intracellular tyrosine residues, triggering the recruitment and phosphorylation of the transcription factor STAT3. Related references: PMID:12133952
• Phosphorylated STAT3 homodimerizes and translocates to the nucleus, where it induces anti-inflammatory gene expression. IL-10RA signaling also suppresses starvation-induced autophagy through STAT3. Related references: PMID:26962683
• IL-10RA is primarily expressed on the surface of hematopoietic cells, including B-cells, T-cells, NK cells, monocytes, and macrophages, but is absent from non-hematopoietic cells like fibroblasts and endothelial cells.
• The protein localizes to the cell membrane and cytoplasm, and it undergoes post-translational modifications such as glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–235) of human IL-10RA, suggesting the antibody may recognize an epitope in this region and is suitable for surface staining of live cells.
• As an conjugate validated for flow cytometry, titration experiments should be considered to optimize the signal-to-noise ratio in the specific experimental system.
• Because IL-10RA expression is restricted to hematopoietic cells, include relevant human cell models (e.g., PBMCs, isolated immune cell subsets) and appropriate isotype controls in FC assays.
• Cell stimulation with IL-10 can influence receptor dynamics; for functional studies, consider comparing resting versus activated conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents—including rare-target sourcing if needed. For IL-10RA/CD210 research, we supply rabbit monoclonal antibodies (such as the conjugate highlighted here) to support immune-regulation studies, anti-inflammatory screening, and cell phenotyping. With comprehensive technical services and consulting available, CamelBio helps bridge your diagnostic development from concept to clinical application.