Rabbit Anti-Human IgG2 (Fc) Monoclonal Antibody for WB, ELISA - P01859

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant gamma 2 Function and Localization

• The IgG2 heavy chain constant region plays a central role in humoral immunity, serving both as B-cell antigen receptor and secreted antibody that neutralizes pathogens and activates complement. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Encoded by the IGHG2 gene (GeneID: 3501), IgG2 is one of four IgG subclasses (IgG1–IgG4) and arises through class-switch recombination from IgM/IgD in activated B lymphocytes.
• Alternative names for the protein include Ig gamma-2 chain C region DOT, TIL, ZIE, and simply Ig gamma-2 chain C region.
• The antibody targets the fragment crystallizable (Fc) region (amino acids 99‑326), which mediates binding to Fcγ receptors on innate immune cells and C1q of the complement cascade.
• IgG2 is specialized for responses to polysaccharide antigens on encapsulated bacteria, with its constant region driving antibody-dependent complement activation.
• Subcellularly, IgG2 is found secreted into extracellular fluids and as a membrane-bound receptor on B cells, anchored by a transmembrane helix.
• Post-translational modifications include N-linked glycosylation and interchain disulfide bonds critical for antibody structure and stability.
• UniProt keywords highlight adaptive immunity, immunoglobulin domain architecture, alternative splicing, and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen (aa 99–326 of human IgG2 Fc) ensures specificity for the constant region, minimizing cross-reactivity with other IgG subclasses.
• For Western blotting, empirically optimize primary antibody dilution and validate with purified human IgG2 or serum samples under reducing and non-reducing conditions.
• In ELISA assays, the antibody may be used as a capture or detection reagent; optimization of coating, blocking, and detection conditions is recommended.
• Because the target exists in both secreted and membrane-anchored forms, adjust sample preparation (e.g., tissue or cell lysates) accordingly for membrane IgG2 detection.
• Always validate performance in your specific sample matrix and experimental system, as glycosylation patterns and matrix effects can influence results.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material supplier, CamelBio provides this anti-human IgG2 (Fc) monoclonal antibody alongside a full portfolio of immunoglobulin reagents for diagnostic assay development. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, we support your projects with reliable materials and technical expertise. Let CamelBio be your bridge from concept to clinic, simplifying sourcing for humoral immunity and antibody isotyping research.