Rabbit anti-Human IgG (Fc) Monoclonal Antibody for Flow Cytometry - P01857

Core Product Specifications and Parameters

Biological Background: Human IgG Fc (IGHG1) Function and Localization

• IGHG1 encodes the constant region of immunoglobulin gamma-1 heavy chain, a key component of IgG1 antibodies. This region defines the isotype and mediates effector functions through the Fc fragment. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• IgG1 molecules are secreted glycoproteins or membrane-bound receptors on B cells. In humoral immunity, membrane-bound Ig serves as the B-cell receptor, and upon antigen binding, triggers B-cell activation and differentiation into plasma cells secreting antibodies. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The Fc region interacts with Fc receptors on innate immune cells and complement C1q, triggering antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent phagocytosis, complement-dependent cytotoxicity (CDC), and inflammatory responses. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The immunoglobulin heavy chain locus undergoes V(D)J recombination, somatic hypermutation, and class-switch recombination to generate diverse antibody repertoires, with IgG1 being the most abundant subclass in human serum.
• The protein contains conserved immunoglobulin domains stabilized by disulfide bonds and is N-glycosylated at Asn-180, critical for proper folding and Fc receptor binding.
• Subcellularly, IgG1 is either membrane-anchored (via a transmembrane domain) on B-cell surfaces or secreted as a soluble antibody; the Fc region mediates transportation across epithelial barriers via the neonatal Fc receptor.
• IGHG1 is located on chromosome 14 within the Ig heavy chain locus and is expressed specifically in B cells and plasma cells; its constant region is swapped during class switching from IgM/IgD.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-330 of human IgG1 Fc, covering the CH2 and CH3 domains, which are highly conserved across IgG1 allotypes. Ensure appropriate controls (e.g., IgG isotype controls) when using this antibody in flow cytometry.
• Typical working concentration for flow cytometry should be optimized by titration; consider using 0.5-5 µg per test for human whole blood or PBMCs.
• This antibody is specific for human IgG Fc; test for cross-reactivity with other species if multi-species experiments are planned. It does not bind to Fab fragments.
• To minimize Fc receptor-mediated background, pre-block cells with human serum or Fc block before staining.
• Use fresh paraformaldehyde fixation (0.5-1%) after staining to preserve fluorescence; avoid methanol as it may denature the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions for diagnostic manufacturers and researchers. This Rabbit anti-Human IgG (Fc) monoclonal antibody is part of our catalog of validated, ready-to-use antibodies for flow cytometry applications in immune monitoring, vaccine development, and therapeutic antibody quantification. Beyond individual reagents, CamelBio provides customized sourcing of antibody pairs, bulk ancillary reagents, and rare-target raw materials to streamline your assay development from concept to clinic. Whether you need optimized monoclonal antibodies, conjugates, or specialty reagents, our integrated one-stop platform ensures quality, traceability, and reliable supply.