Rabbit anti-Human IgG (Fc) Monoclonal Antibody for Flow Cytometry - P01857

Core Product Specifications and Parameters

Biological Background: IgG (Fc) Function and Localization

• The target is the constant region (Fc) of human immunoglobulin gamma-1 heavy chain (IgG1), encoded by the IGHG1 gene, corresponding to amino acids 100-330 of the mature protein (UniProt P01857). This region mediates effector functions via interaction with Fc receptors and complement.
• Immunoglobulin heavy chains are integral to adaptive immunity: membrane-bound Ig serves as B-cell receptor, while secreted antibodies neutralize pathogens. The Fc region of IgG1 specifically triggers antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity, and phagocytosis (PubMed:17576170, PubMed:20176268, PubMed:22158414). Related references: PMID:17576170 PMID:20176268 PMID:22158414
• IgG1 consists of two identical heavy chains disulfide-linked to two light chains; each heavy chain contains one variable domain (encoded by V, D, J segments) and three constant domains (CH1, CH2, CH3). The CH2 and CH3 domains form the Fc fragment targeted by this antibody.
• The IGHG1 gene is part of the immunoglobulin heavy chain locus on chromosome 14, and class-switch recombination from IgM/IgD to IgG1 occurs in activated B cells, shaping the antibody repertoire.
• IgG1 is the most abundant antibody isotype in human serum and is crucial for long-term humoral immunity, often used as a therapeutic antibody scaffold.
• Subcellular localization: IgG1 heavy chains are secreted as antibodies or displayed on the surface of B cells as membrane-bound immunoglobulins (transmembrane form).
• Post-translational modifications include disulfide bond formation and N-glycosylation at the conserved CH2 domain, which is essential for Fc receptor binding and stability.
• Keywords: adaptive immunity, secreted, cell membrane, glycoprotein, immunoglobulin domain, disulfide bond.

Experimental Guidance and Technical Tips

• The antibody is conjugated to , a photostable fluorophore with excitation/emission maxima ~590/617 nm, compatible with standard flow cytometers. Ensure appropriate instrument settings and compensation.
• For flow cytometry, titrate the antibody to determine optimal staining concentration (typically 0.5-5 µg per 10^6 cells) using positive and negative control cells expressing human IgG (e.g., human B cells or cell lines expressing membrane IgG).
• Since the antibody recognizes the Fc region, it may bind to free IgG in serum or culture medium; wash cells thoroughly to reduce background.
• This clone may be used to detect surface IgG on human B cells, or for identification of IgG-coated particles or immune complexes in research settings.
• Avoid harsh fixation/permeabilization that may alter the Fc epitope; mild paraformaldehyde fixation (1-4%) is generally compatible.
• Use Fc receptor blocking reagents when staining cells with high FcγR expression to prevent non-specific binding.
• Consider validating cross-reactivity with other IgG subclasses (IgG2, IgG3, IgG4) if needed, as the Fc region sequences share high homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research institutes, and laboratories with comprehensive IVD raw-material procurement. This anti-human IgG (Fc) monoclonal antibody complements research in immunology, antibody engineering, and B-cell biology. In addition to validated primary antibodies, CamelBio can supply optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, streamlining your workflow from early-stage development to clinical application.