Rabbit anti-Human IgG (Fc) Monoclonal Antibody for FC - P01857

Core Product Specifications and Parameters

Biological Background: IgG1 Fc Constant Region Function and Localization

• IgG1 is the constant region of immunoglobulin heavy chains, mediating effector functions of humoral immunity via its Fc fragment. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• As part of the Ig molecule, the Fc region interacts with Fc receptors on innate immune cells and complement components to trigger antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and complement-dependent cytotoxicity (CDC).
• The constant region defines the IgG isotype; class-switch recombination enables B cells to produce IgG1, involved in adaptive immunity.
• Subcellular localization: Secreted and cell membrane-bound forms exist (membrane-bound Ig serves as B cell receptor).
• Post-translational modifications include glycosylation, disulfide bond formation, and alternative splicing generating membrane-bound and secreted isoforms.
• Keywords: Immunoglobulin, IgG1, Adaptive immunity, Glycoprotein, Membrane, Secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the Fc region (aa 100-330) of human IgG1, ensuring specific targeting of the constant domain. For flow cytometry (FC), consider validating antibody performance with relevant human cell lines expressing IgG1, such as B lymphoblastoid cells or IgG1-expressing hybridomas.
• conjugate is suitable for direct fluorescent detection in FC; ensure proper isotype controls and titration experiments.
• Since the antibody targets the Fc region, it may also recognize other IgG subclasses or Ig forms with similar Fc domains; cross-reactivity should be evaluated in the experimental system.
• Fixation and permeabilization protocols may affect epitope recognition; optimize staining conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, technical services, and consulting to support your diagnostic development from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers and manufacturers working with human IgG1 Fc targets, we provide reliable access to high-quality reagents and customized supply chains.