Rabbit anti-Human IgE Monoclonal Antibody for FC - P01854

Core Product Specifications and Parameters

Biological Background: IgE Function and Localization

• Human IgE serves as the constant region of immunoglobulin heavy chains, functioning in humoral immunity by acting as both membrane-bound antigen receptor on B cells and secreted antibody that eliminates antigens. Related references: PMID:20176268, PMID:22158414, PMID:17576170
• Secreted IgE (Fc region) triggers immediate hypersensitivity and allergic reactions by binding to high-affinity receptor FCER1A on mast cells, basophils, and eosinophils, causing degranulation and release of inflammatory mediators; it also mediates anti-parasitic and anti-venom responses. Related references: PMID:2167225, PMID:25629393, PMID:33840121, PMID:7544003, PMID:8114916, PMID:8551243
• Through FCER2 receptor, IgE immune complexes activate macrophage intracellular killing of parasites (L-arginine-nitric oxide pathway) and mediate antibody-dependent cellular cytotoxicity (ADCC) against tumor cells, as well as macrophage reprogramming toward a proinflammatory antitumor state. Related references: PMID:7544003, PMID:30956175
• Membrane-bound IgE isoforms constitute the B cell receptor (BCR): the long isoform (mIgE-L) promotes B cell survival and plasma cell differentiation upon antigen binding, while the short isoform (mIgE-S) provides sustained negative regulatory signals to mature B cells.
• IgE is expressed primarily in B lymphocytes upon stimulation with IL-4 and CD40, consistent with its role in allergic responses and class switching.
• Subcellular localization includes secretion for the soluble form and cell membrane anchoring for the transmembrane isoforms, enabling both systemic distribution and cell-surface receptor function.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for proper folding and stability of the immunoglobulin domains.
• IgE is classified under adaptive immunity, inflammatory response, and immunoglobulin domain-containing proteins, highlighting its role in both protective and pathological immune reactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the constant region (CH2-CH4 domains) of human IgE, ensuring specific detection without cross-reactivity to other immunoglobulin isotypes.
• This conjugate is suitable for flow cytometric detection of surface IgE on B cells or basophils; titration may be required to optimize signal-to-noise ratio.
• For intracellular staining of IgE in plasma cells, consider fixation and permeabilization protocols.
• Validate performance in your specific sample matrix and consider using appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-human IgE antibody supports critical research in allergy, immunology, and infectious disease, and CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow. Partner with CamelBio for reliable, scalable solutions across the IVD development pipeline.