Rabbit anti-Human IgA1 Monoclonal Antibody for FC - P01876

Core Product Specifications and Parameters

Biological Background: IgA1 Function and Localization

• IGHA1 encodes the constant region of IgA1 heavy chain, a major immunoglobulin class in mucosal secretions. Related references: PMID:2241915
• IgA1 is the predominant IgA subclass in human serum and mucosal secretions, playing a key role in humoral immunity at barrier surfaces.
• In B lymphocytes, membrane-bound IgA1 serves as an antigen receptor, triggering clonal expansion and differentiation into plasma cells. Related references: PMID:20176268, PMID:22158414
• Secreted IgA1 mediates the effector phase of humoral immunity by binding antigens and facilitating their elimination.
• The antigen binding site is formed by the variable domain of one heavy chain paired with its associated light chain; V(D)J rearrangement and somatic hypermutation drive affinity maturation. Related references: PMID:17576170, PMID:20176268
• Subcellular localization: Secreted as a soluble antibody and also expressed as a membrane-bound form on the surface of B cells.
• Post-translational modifications include glycosylation, disulfide bond formation, and alternative splicing, contributing to structural and functional diversity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 99–353 of human IgA1) covers the constant region, likely conferring specificity for IgA1 without cross-reacting with other immunoglobulin classes.
• For flow cytometry, titrate the conjugate to determine the optimal staining concentration that minimizes background and maximizes signal-to-noise ratio.
• Include appropriate isotype controls and ensure proper compensation when using this antibody in multi-color FC panels.
• Validate reactivity in the intended sample system (e.g., human PBMCs or tissue-derived single-cell suspensions) before large-scale experiments.
• As a rabbit monoclonal antibody, expect high affinity and batch-to-batch consistency; however, always confirm lot-specific performance for critical assays.
• The antibody is validated for human targets; if used with non-human species, cross-reactivity should be tested experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This Rabbit anti-Human IgA1 mAb enables flow-cytometric detection of IgA1+ cells in mucosal immunity studies. As your sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material acquisition to accelerate your IgA1-related research and assay development.