Rabbit anti-Human Ig κ Light Chain Monoclonal Antibody for Flow Cytometry - P01834

Core Product Specifications and Parameters

Biological Background: Ig κ Light Chain Function and Localization

• The immunoglobulin kappa constant region (IGKC) is the constant part of kappa light chains, which together with heavy chains form functional antibodies (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268, PMID:22158414
• Membrane-bound immunoglobulins act as B cell receptors, triggering clonal expansion and differentiation upon antigen binding (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268, PMID:22158414
• Secreted antibodies mediate humoral immunity by eliminating bound antigens (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268, PMID:22158414
• The variable domain of one heavy chain pairs with a light chain variable domain to form the antigen-binding site, with affinity maturation achieved through somatic hypermutation (PubMed:17576170, PubMed:20176268). Related references: PMID:17576170, PMID:20176268
• IGKC is found both secreted and on the cell membrane, playing a pivotal role in adaptive immunity.
• The protein contains an immunoglobulin domain and is stabilized by a disulfide bond.
• It is also known by alternative names such as Km, HCAK1, and IGKCD.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length constant region (aa 1-107), suggesting that the antibody can recognize all kappa light chains irrespective of variable region sequence.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Use an appropriate isotype control conjugated to the same fluorophore () to assess non-specific binding.
• Consider cell surface staining for live cells or fixation/permeabilization protocols if intracellular detection is required.
• is a far-red fluorophore; ensure compatibility with your flow cytometer's laser and filter configuration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research institutes by offering a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-human Ig κ Light Chain clone. Whether you need bulk ancillary reagents or assistance with rare target sourcing, our supply chain solutions are designed to support your workflow from concept to clinic. Contact us to explore how CamelBio can accelerate your assay development with reliable, research-grade reagents.