Rabbit anti-Human Ig κ Light Chain Monoclonal Antibody for FC - P01834

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Kappa Constant (IGKC) Function and Localization

• IGKC encodes the constant region of immunoglobulin kappa light chains, an essential component of antibody molecules. It is a member of the immunoglobulin gene family.
• Antibodies exist as membrane-bound B cell receptors or secreted glycoproteins. Upon antigen recognition, membrane-bound immunoglobulins trigger B lymphocyte clonal expansion and differentiation into antibody-secreting plasma cells, while secreted forms mediate antigen elimination. Related references: PMID:20176268 PMID:22158414
• Each immunoglobulin contains two antigen-binding sites formed by the variable domains of one heavy and one light chain. These variable domains are produced by V(D)J recombination and can undergo somatic hypermutation, enabling affinity maturation after antigen exposure. Related references: PMID:17576170 PMID:20176268
• IGKC is found on the cell membrane as part of the B cell receptor complex and is also secreted as a soluble antibody light chain component.
• The protein features an immunoglobulin-like domain, contains conserved disulfide bonds, and has a theoretical molecular weight of approximately 11.7 kDa (UniProt).

Experimental Guidance and Technical Tips

• Titrate the antibody to determine the optimal concentration for flow cytometry, ensuring a clear separation between positive and negative populations.
• Include appropriate isotype control antibodies conjugated to the same fluorochrome to assess non-specific binding.
• Validate performance using human peripheral blood mononuclear cells (PBMCs) or relevant B cell lines to confirm specific staining of surface or intracellular IGKC.
• For intracellular staining, consider fixation and permeabilization protocols compatible with fluorochrome stability and epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by offering validated antibodies, bulk ancillary reagents, and rare target sourcing—backed by technical services and regulatory consulting. This anti-IGKC monoclonal antibody, conjugated to , supports flow cytometry applications in immunological diagnostics and B cell research. If you require customized antibody development, bulk supply, or complementary reagents, our team can tailor solutions to your development pipeline.