Rabbit anti-Human Ig κ Light Chain Monoclonal Antibody for FC - P01834

Core Product Specifications and Parameters

Biological Background: Ig κ Light Chain Function and Localization

• The immunoglobulin kappa constant region (IGKC) serves as the constant region of immunoglobulin light chains, playing a critical role in the effector functions of antibodies. Related references: PMID:20176268, PMID:22158414
• Immunoglobulins are produced by B lymphocytes; membrane-bound forms act as antigen receptors, while secreted forms mediate humoral immunity by neutralizing and eliminating antigens.
• The antigen binding site is formed by the pairing of a heavy chain variable domain with a light chain variable domain; V(D)J recombination and somatic hypermutation enable affinity maturation for specific antigens. Related references: PMID:17576170, PMID:20176268
• IGKC exists in membrane-bound form as part of the B cell receptor and is secreted in large quantities by plasma cells, consistent with its subcellular localization at the cell membrane and in the extracellular space.
• Disulfide bonds stabilize the immunoglobulin domain structure, a hallmark of this protein family.
• Multiple allelic variants (Km allotypes) exist, including Ig kappa chain C region AG, CUM, EU, OU, ROY, and TI.
• IGKC has been extensively characterized via direct protein sequencing and proteomics, and is a component of the human reference proteome.

Experimental Guidance and Technical Tips

• Given that the immunogen spans the constant region (aa 1-107), this antibody is expected to recognize all human kappa light chain-containing antibodies irrespective of variable region specificity; validate this pan-kappa reactivity in your experimental system.
• For flow cytometry, use appropriate Fc receptor blocking to reduce non-specific binding, and include an -conjugated isotype control.
• Consider titrating the antibody to determine optimal staining concentration for your cell type; signal may vary with cell surface immunoglobulin expression levels.
• If staining secreted immunoglobulins or intracellular kappa chains, optimize permeabilization and fixation steps.
• Since the protein has a small molecular weight (~11 kDa), ensure SDS-PAGE and Western blot conditions are suitable for low-molecular-weight proteins if used for other applications, though this antibody is validated for FC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For immunoglobulin targets like Ig kappa light chain, we support diagnostic developers with consistent-quality reagents essential for immune status monitoring and hematological malignancy detection. Our supply chain capabilities also include rare-target raw-material sourcing to accelerate your assay development from concept to clinic.