Rabbit anti-Human Ig κ Light Chain mAb - P01834

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Kappa Constant Function and Localization

• The immunoglobulin kappa constant region (IGKC) is the constant part of the light chain in antibodies, forming both membrane-bound B cell receptors and secreted immunoglobulins essential for humoral immunity. Related references: PMID:20176268, PMID:22158414
• Antigen binding is achieved through pairing of the variable domain of one light chain with a heavy chain, and affinity maturation occurs via V(D)J recombination and somatic hypermutation upon antigen exposure. Related references: PMID:17576170, PMID:20176268
• Alternative names for IGKC include HCAK1, IGKCD, and Km, with several allelic variants (e.g., AG, CUM, EU, OU, ROY, TI) reported.
• The protein is expressed on the cell membrane as part of the B cell receptor complex and is secreted by plasma cells into the bloodstream.
• Post-translational modifications include a conserved disulfide bond that stabilizes the immunoglobulin domain fold, contributing to antibody structural integrity.
• It plays a key role in adaptive immunity and is used as a marker for B cell lineage in immunophenotyping and diagnostics.

Experimental Guidance and Technical Tips

• As a fluorophore-conjugated antibody, direct immunofluorescence staining for flow cytometry is possible without a secondary antibody. Titrate to determine optimal staining concentration for your cell type.
• Human peripheral blood mononuclear cells (PBMCs) or B cell lines are suitable positive controls for surface Ig κ light chain detection.
• Include appropriate isotype control and Fc receptor blocking to minimize non-specific binding.
• Permeabilization is required for intracellular staining of secreted Ig κ light chain in plasma cells.
• Ensure complete dissociation of cell aggregates prior to acquisition for accurate flow cytometric analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic developers from concept to clinic. For research targeting human immunoglobulin light chains, we provide high-quality antibodies like this -conjugated rabbit anti-Ig κ light chain mAb for flow cytometry applications. Our sourcing capabilities also cover rare targets and custom development to streamline your assay workflows.