Rabbit anti-Human Ig κ Light Chain mAb for FC - P01834

Core Product Specifications and Parameters

Biological Background: Human Ig κ Light Chain Function and Localization

• The human immunoglobulin kappa constant region (IGKC) is the constant domain of the kappa light chain, essential for antibody structure and effector functions.
• Immunoglobulins are B-cell-produced glycoproteins; membrane-bound forms act as B-cell receptors, while secreted antibodies mediate humoral immunity (PubMed:20176268, 22158414). Related references: PMID:20176268 PMID:22158414
• Antigen-binding specificity is determined by the variable domain, which undergoes V(D)J recombination and somatic hypermutation for affinity maturation (PubMed:17576170, 20176268). Related references: PMID:17576170 PMID:20176268
• IGKC is found in both secreted antibodies and as membrane-bound B-cell receptors (SUBCELLULAR LOCATION: Secreted, Cell membrane).
• The constant region forms an immunoglobulin domain stabilized by a conserved disulfide bond (KEYWORDS: Disulfide bond, Immunoglobulin domain).
• It has been extensively studied, with multiple 3D structures available and proteomic identification confirmed (KEYWORDS: 3D-structure, Proteomics identification).
• IGKC is part of the reference proteome and is linked to adaptive immunity (KEYWORDS: Adaptive immunity, Reference proteome).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-107 of the human Ig kappa constant region, suggesting recognition of the full-length constant domain in native and denatured forms.
• Designed for flow cytometry (FC); use direct immunofluorescence staining with appropriate titrations to minimize background.
• As a directly conjugated rabbit monoclonal antibody, it enables one-step staining; validate in human B-cell lines or peripheral blood mononuclear cells (PBMCs).
• Since the target is both secreted and membrane-bound, consider cell permeabilization protocols if intracellular detection is required.
• Compatibility with other applications (e.g., IHC, ELISA) is possible but not validated; independent verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD raw materials, offering validated antibodies, bulk reagents, and rare-target sourcing for diagnostic development. This anti-human Ig kappa light chain conjugate supports B-cell immunophenotyping and immunoglobulin research, complementing our broader portfolio of immune-related reagents. Partner with us to accelerate your assay development from concept to clinic.