Rabbit anti-Human IFN-gamma Monoclonal Antibody for Flow Cytometry (Intracellular) - P01579

Core Product Specifications and Parameters

Biological Background: Interferon gamma Function and Localization

• Interferon gamma (IFN-gamma), also known as immune interferon, is a type II interferon encoded by the IFNG gene. It plays critical roles in antimicrobial, antiviral, and antitumor immunity by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093 PMID:8666937
• IFN-gamma signals primarily through the JAK-STAT pathway after binding to its receptor IFNGR1. This interaction triggers the activation of JAK2, JAK1, and STAT1, leading to STAT1 nuclear translocation and transcription of downstream target genes, including the transcription factor IRF1. Related references: PMID:8349687 PMID:16914093
• In the class I antigen presentation pathway, IFN-gamma induces the replacement of standard proteasome subunits with immunoproteasome subunits, increasing the quantity, quality, and repertoire of peptides loaded onto MHC class I molecules. It also enhances peptide generation efficiency by upregulating the PA28 activator. Related references: PMID:8666937 PMID:8163024 PMID:11112687
• For class II antigen presentation, IFN-gamma upregulates MHC II complexes by promoting the expression of cathepsins B, H, and L. Related references: PMID:7729559
• The protein is secreted and primarily released from activated T lymphocytes. It participates in regulating hematopoietic stem cell development, quiescence, and differentiation.
• UniProt keywords for IFN-gamma include antiviral defense, cytokine, glycoprotein, secreted, and pharmaceutical, reflecting its structural and functional properties.

Experimental Guidance and Technical Tips

• When performing intracellular flow cytometry, ensure proper fixation and permeabilization protocols, as IFN-gamma is a secreted cytokine that requires intracellular staining for detection.
• The immunogen corresponds to the mature secreted form (amino acids 24-166), suggesting the antibody likely recognizes the processed, biologically active cytokine.
• Consider using appropriate positive controls, such as activated human T cells or PBMCs stimulated with PMA/ionomycin, to validate staining specificity.
• To minimize non-specific binding, titrate the antibody and include appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. For IFN-gamma research and immunoassay development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our supply network also supports sourcing rare-target raw materials, accelerating your projects from concept to clinic.