Rabbit anti-Human IFN-alpha/beta R2 Monoclonal Antibody for Flow Cytometry - P48551

Core Product Specifications and Parameters

Biological Background: Interferon alpha/beta receptor 2 Function and Localization

• IFNAR2 (interferon alpha/beta receptor 2), encoded by the IFNAR2 gene (aliases: IFNABR, IFNARB), is a subunit of the type I interferon receptor. Multiple splice variants yield proteins with molecular weights of approximately 58 kDa, 37 kDa, and 27 kDa.
• Together with IFNAR1, IFNAR2 forms a heterodimeric receptor that binds type I interferons, including IFN-α, -β, -ε, -ω, and -κ. Related references: PMID:10049744 PMID:10556041 PMID:21854986
• Ligand binding induces receptor dimerization, bringing the intracellular domains into proximity and triggering transphosphorylation of associated Janus kinases (JAK1 on IFNAR2, TYK2 on IFNAR1). Related references: PMID:10556041 PMID:11682488 PMID:12105218
• Activated JAKs phosphorylate specific tyrosine residues on the intracellular tails of IFNAR1 and IFNAR2, creating recruitment sites for STAT1 and STAT2. Phosphorylated STATs dimerize and translocate to the nucleus to drive transcription of interferon-stimulated genes (ISGs). Related references: PMID:11682488 PMID:12105218 PMID:21854986
• A naturally occurring soluble isoform of IFNAR2 (isoform 3) is secreted and acts as a potent inhibitor of type I IFN signaling, serving as a regulatory decoy receptor.
• The receptor is predominantly localized to the cell membrane but is also found in secreted form; it is widely expressed in blood cells and certain tumor cell lines, consistent with its role in innate antiviral immunity.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant protein immunogen corresponding to amino acids 28-237 of human IFNAR2. Researchers may consider using a similar immunogen strategy for generating antibodies against the extracellular domain.
• The antibody is validated for flow cytometry (FC) with human samples. For other applications, we recommend performing pilot experiments to determine optimal working concentrations and fixation/permeabilization conditions.
• Given the existence of multiple splice isoforms, confirm the recognized isoform(s) by western blot or other assays if needed, and consider the potential presence of soluble IFNAR2 in sample supernatants.

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CamelBio streamlines the sourcing of IVD raw materials by supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For interferon research and type I IFN pathway studies, we offer one-stop access to critical raw materials and technical consulting from concept to clinic. Let our procurement expertise support your next-generation assay development.