Rabbit anti-Human IDO1 Monoclonal Antibody for Flow Cytometry - P14902

Core Product Specifications and Parameters

Biological Background: IDO1 Function and Localization

• IDO1 (Indoleamine 2,3-dioxygenase 1) is a heme-containing enzyme that catalyzes the oxidative cleavage of tryptophan, the first and rate-limiting step in the kynurenine pathway. Related references: PMID:17671174 PMID:18026683

• It plays a central role in immune tolerance: local tryptophan depletion inhibits T-cell proliferation, while downstream catabolites such as kynurenines induce T-cell apoptosis and promote the differentiation of regulatory T cells. Related references: PMID:25691885

• IDO1 acts as a potent suppressor of anti-tumor immunity, allowing tumors to evade immune surveillance. Related references: PMID:14502282 PMID:23103127 PMID:25157255

• It limits the growth of intracellular pathogens by depriving them of tryptophan. Related references: PMID:25691885

• IDO1 contributes to fetal-maternal tolerance, protecting the semiallogeneic fetus from maternal immune rejection. Related references: PMID:25691885

• The protein is primarily localized to the cytoplasm and cytosol.

• IDO1 is constitutively expressed in mature dendritic cells of lymphoid organs, certain epithelial cells, and placental endothelial cells. It is weakly expressed or absent in most normal tissues but is highly inducible. Over 50% of human tumors show IDO1 expression, often correlating with poor clinical outcome.

• As a dioxygenase, IDO1 contains heme and iron cofactors and belongs to the oxidoreductase family.

Experimental Guidance and Technical Tips

• This -conjugated antibody is optimized for direct intracellular flow cytometry and can be used without the need for a secondary detection reagent.
• For intracellular staining, cells require fixation and permeabilization prior to antibody incubation; follow standard intracellular staining protocols.
• Validate the antibody in a well-characterized positive control system (e.g., IDO1-expressing dendritic cells or tumor cell lines) and include appropriate isotype and fluorescence-minus-one controls.
• Titrate the antibody to determine the optimal concentration for your specific assay and sample type, as staining conditions may vary.
• Consider using this antibody in combination with other markers for multiparametric immune profiling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, research laboratories, and IVD developers with a comprehensive range of high-quality raw materials, including this anti-IDO1 monoclonal antibody. Whether you need validated antibody pairs, optimized monoclonal/polyclonal reagents, or bulk ancillary reagents for immuno-oncology and immune tolerance research, CamelBio streamlines procurement from concept to clinic. Contact us to discuss sourcing rare-target raw materials, custom conjugations, or full workflow support for your next diagnostic project.