Rabbit anti-Human ICAM2/CD102 Monoclonal Antibody for Flow Cytometry - P13598

Core Product Specifications and Parameters

Biological Background: ICAM2/CD102 Function and Localization

• Intercellular adhesion molecule 2 (ICAM2, CD102) is a cell surface glycoprotein belonging to the immunoglobulin superfamily, functioning as a ligand for integrin LFA-1 (ITGAL:ITGB2) and dendritic cell receptor DC-SIGN (CD209).
• ICAM2 mediates leukocyte cell-cell adhesion on resting cells, ensuring critical immune functions such as antigen presentation, lymphocyte recirculation, and immune surveillance. Related references: PMID:10077629, PMID:1676048, PMID:2497351
• Through interaction with DC-SIGN, ICAM2 triggers transendothelial migration of dendritic cell precursors, facilitating immune cell trafficking from blood into peripheral and lymphoid tissues. Related references: PMID:11017109
• Subcellularly, ICAM2 localizes to the cell membrane and is enriched on microvilli, consistent with its role in cellular projections for adhesion.
• Constitutively expressed on leukocytes, including resting lymphocytes and monocytes, but absent in neutrophils; also highly expressed on vascular endothelial cells and lymphatic vessels in tonsils. Related references: PMID:1676048, PMID:11017109
• Structurally, ICAM2 contains immunoglobulin-like domains, disulfide bonds, and a transmembrane region; its mature protein has a predicted molecular weight of 30.6 kDa (approximately 31 kDa observed).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25-223), ensuring the antibody can recognize surface-expressed ICAM2 on live cells in flow cytometry applications.
• As an conjugate, this antibody is ready-to-use for direct flow cytometry without secondary detection; consider titrating to optimize the signal-to-noise ratio for your specific cell system.
• This antibody is specific for human ICAM2; cross-reactivity with other species has not been reported, so validate in the relevant sample system if non-human models are used.
• For intracellular staining protocols requiring permeabilization, confirm that the epitope remains accessible under the chosen fixation and permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop sourcing solution for IVD raw materials. This anti-ICAM2 monoclonal antibody supports immune cell adhesion and migration studies, complementing our portfolio of validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing. From concept to clinic, we streamline procurement with integrated technical and supply chain support for your project needs.