Rabbit anti-Human ICAM2/CD102 Monoclonal Antibody for FC - P13598

Core Product Specifications and Parameters

Biological Background: ICAM2/CD102 Function and Localization

• ICAM2 (Intercellular adhesion molecule 2), also known as CD102, is a cell surface glycoprotein of the immunoglobulin superfamily, characterized by immunoglobulin domains, disulfide bonds, and glycosylation.
• It functions as a major ligand for integrin ITGAL:ITGB2 (LFA-1) on leukocytes, mediating cell-cell adhesion critical for antigen presentation, lymphocyte recirculation, and immune surveillance. Related references: PMID:10077629, PMID:1676048, PMID:2497351
• Additionally, ICAM2 serves as an endothelial ligand for dendritic cell CD209 (DC-SIGN), triggering transendothelial migration of dendritic cell precursors from blood to tissues. Related references: PMID:11017109
• ICAM2 is constitutively expressed on leukocytes (resting lymphocytes and monocytes, but not neutrophils) and endothelial cells, including vascular and lymphatic vessels.
• Subcellularly, it localizes to the cell membrane and microvilli, consistent with its role in cellular adhesion and interactions.
• Key post-translational modifications include disulfide bond formation and N-linked glycosylation, which contribute to its structural integrity and binding functions.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 25-223) of human ICAM2, indicating that the antibody likely recognizes an epitope accessible on live cells, making it suitable for flow cytometry.
• As a direct conjugate, this antibody can be used without a secondary antibody; titrate carefully to determine the optimal concentration for your cell type and avoid non-specific binding.
• Validate staining performance on known ICAM2-expressing human cell lines (e.g., leukocytes or endothelial cells) and include appropriate isotype controls.
• Consider that receptor engagement (e.g., by LFA-1 or DC-SIGN) might influence staining patterns; ensure cells are in a resting state for consistent baseline detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop sourcing for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibodies, antigen pairs, and bulk reagents. This anti-ICAM2 monoclonal antibody aids immunology studies focusing on cell adhesion, immune cell trafficking, and endothelial interactions. Our technical team can assist with customized procurement, including rare-target raw materials and assay optimization, from concept to clinic.