Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular Adhesion Molecule 1, CD54) is an immunoglobulin superfamily glycoprotein that binds the leukocyte integrins LFA-1 (ITGAL:ITGB2) and Mac-1 (ITGAM:ITGB2), mediating T-cell adhesion and neutrophil-endothelial interactions (PubMed:3086451, PubMed:3340213, PubMed:38195629, PubMed:1980124).
  Related references:
  PMID:3086451, PMID:3340213, PMID:38195629, PMID:1980124

• Upon ICAM-1 engagement, downstream signaling through ARHGEF26/SGEF and RHOG drives the assembly of endothelial apical cups, a critical step in leukocyte trans-endothelial migration (PubMed:17875742).
  Related references:
  PMID:17875742

• ICAM-1 functions as the cellular receptor for major group rhinoviruses (A and B) and Coxsackievirus A21, facilitating viral entry into host cells.

• Through its interaction with MUC1, ICAM-1 promotes epithelial cell aggregation (PubMed:11173916).
  Related references:
  PMID:11173916

• ICAM-1 is constitutively expressed on vascular endothelial cells, thymic epithelium, fibroblasts, and at low levels on resting peripheral blood leukocytes; expression is strongly upregulated by inflammatory cytokines (PubMed:3086451).
  Related references:
  PMID:3086451

• The protein localizes primarily to the plasma membrane as a type I transmembrane protein, with a shed soluble form detected in serum.

• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation; during HHV-8 infection, the viral E3 ligase MIR2 mediates ubiquitination and degradation of ICAM-1 to subvert immune recognition.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 28-480), enabling recognition of native ICAM-1 on live cells; direct conjugation to allows one-step flow cytometry.
• ICAM-1 expression is low on resting leukocytes; consider activating cells with PMA/ionomycin or cytokines to achieve robust staining.
• Titrate the antibody in your experimental system to determine the optimal concentration for flow cytometry, and include appropriate isotype control and viability dye.
• Cross-reactivity has been confirmed with human; validation for other species is not reported—verify if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. This anti-ICAM-1 antibody supports immunological and infectious disease research, and we can assist with customized procurement of rare-target reagents. Reach out to streamline your raw material supply chain.