Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54 or major group rhinovirus receptor, is encoded by the ICAM1 gene and belongs to the immunoglobulin superfamily.
• It functions as a ligand for the integrins LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2), mediating leukocyte adhesion and trans-endothelial migration (PubMed:3086451, PubMed:3340213, PubMed:38195629, PubMed:1980124).
  Related references: PMID:3086451, PMID:3340213, PMID:38195629, PMID:1980124
• During leukocyte trans-endothelial migration, ICAM-1 engagement promotes endothelial apical cup assembly via ARHGEF26 and RHOG activation (PubMed:17875742).
  Related references: PMID:17875742
• ICAM-1 also mediates cell aggregation in epithelial cells through interaction with MUC1 (PubMed:11173916).
  Related references: PMID:11173916
• It acts as a receptor for major group rhinoviruses (A-B) and Coxsackievirus A21, facilitating viral entry into host cells.
• Kaposi's sarcoma-associated herpesvirus (KSHV) encodes an E3 ubiquitin ligase MIR2 that degrades ICAM-1, potentially evading immune detection.
• ICAM-1 is widely expressed on non-hematopoietic cells (vascular endothelial cells, epithelial cells, fibroblasts) and hematopoietic cells (tissue macrophages, activated T cells, dendritic cells), with low expression on peripheral blood leukocytes (PubMed:3086451).
  Related references: PMID:3086451
• The protein is a transmembrane glycoprotein with multiple immunoglobulin-like domains, disulfide bonds, and potential phosphorylation and ubiquitination sites.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a far-red fluorophore with excitation suitable for common flow cytometers equipped with a 633–635 nm laser; no secondary antibody is needed.
• The immunogen encompasses the extracellular domain (amino acids 28–480), making the antibody suitable for detecting surface-expressed ICAM-1 on live, unfixed cells in flow cytometry applications.
• Titrate the antibody in your specific experimental system to determine the optimal staining concentration, and include appropriate isotype controls and Fc receptor blocking steps to minimize nonspecific binding.
• For best results, protect the conjugate from light and avoid repeated freeze-thaw cycles; store undiluted aliquots at recommended temperatures.
• Confirm target expression in the cell population of interest and validate staining specificity using a blocking peptide or cells with known ICAM-1 knockout, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and labs, offering one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For researchers studying cell adhesion and viral entry mechanisms, this ICAM-1/CD54 antibody provides a high-quality flow cytometry tool within a broader portfolio that also supports rare target raw-material sourcing. From concept to clinic, CamelBio’s integrated supply chain helps accelerate your assay development without compromising on quality.