Rabbit anti-Human HER2/ErbB2 Monoclonal Antibody for Flow Cytometry - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• Receptor tyrosine-protein kinase erbB-2 (HER2/ErbB2), also known as proto-oncogene Neu, p185erbB2, or CD340, is a member of the epidermal growth factor receptor family.
• Functions as an essential component of neuregulin-receptor complexes; requires coreceptors for ligand binding and does not interact with neuregulins alone. GP30 is a potential ligand.
• Regulates outgrowth and stabilization of peripheral microtubules via the MEMO1-RHOA-DIAPH1 signaling pathway, leading to GSK3B inhibition and subsequent membrane localization of APC and CLASP2 for microtubule capture.
• In the nucleus, acts as a transcriptional regulator: binds to the PTGS2/COX-2 promoter (5'-TCAAATTC-3') and activates its transcription; also implicated in CDKN1A transcriptional activation involving STAT3 and SRC.
• Activates rRNA gene transcription by RNA polymerase I, enhancing protein synthesis and cell growth.
• Subcellular localization: primarily at the cell membrane, including ruffle membranes; also detected in early endosomes, cytoplasm (perinuclear), and nucleus.
• Post-translational modifications include phosphorylation (phosphoprotein), glycosylation (glycoprotein), and disulfide bond formation; the protein is a transmembrane tyrosine kinase with ATP-binding activity.
• Expressed in various tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney, and mouth.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain of HER2/ErbB2 (amino acids 23-652), which is exposed on the cell surface; this design may be advantageous for flow cytometry applications detecting surface expression.
• For flow cytometry, consider titrating the antibody to determine optimal staining concentration. Include appropriate isotype controls and validate reactivity in your specific cell lines or tumor samples.
• This antibody is validated for flow cytometry; performance in other applications such as immunohistochemistry or Western blotting has not been assessed by the manufacturer and should be tested experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio covers key oncology targets such as HER2/ErbB2, supporting immunoassay development from concept to clinic. For rare or custom targets, we offer specialized sourcing and technical consulting to accelerate your diagnostic projects.