Rabbit anti-Human HER2/ErbB2 Monoclonal Antibody for Flow Cytometry - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• HER2/ErbB2 is a receptor tyrosine-protein kinase that functions within cell surface receptor complexes, requiring a coreceptor for ligand binding. It is an essential component of the neuregulin receptor complex, although neuregulins do not directly interact with it.
• The protein regulates microtubule outgrowth and stabilization via the MEMO1-RHOA-DIAPH1 signaling axis, which inhibits GSK3B and promotes membrane localization of APC and MACF1, critical for microtubule capture.
• In the nucleus, HER2 acts as a transcriptional regulator: it binds to the PTGS2/COX-2 promoter to activate transcription, and it participates in the transcriptional activation of CDKN1A in concert with STAT3 and SRC.
• It is also involved in rRNA gene transcription by RNA polymerase I, thereby enhancing protein synthesis and cell growth.
• Subcellularly, HER2 localizes to the cell membrane, ruffle membrane, early endosomes, cytoplasm (perinuclear region), and nucleus, reflecting its dual signaling and transcriptional roles.
• Expression is observed in a variety of tumor tissues, including primary breast, small bowel, esophageal, kidney, and oral cancers, underscoring its oncogenic significance.
• Key structural features include a signal peptide, a large extracellular domain, a single transmembrane helix, and an intracellular tyrosine kinase domain. Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• This antibody is suitable for flow cytometry (FC) and recognizes the extracellular domain of human HER2. Use appropriate HER2-positive cell lines (e.g., SK-BR-3, BT-474) for positive control, and HER2-negative lines (e.g., MCF-7) as negative control.
• For cell surface staining, harvest live cells and perform staining without fixation/permeabilization to preserve the extracellular epitope. Include an isotype control and Fc block to minimize non-specific binding.
• Titrate the antibody to determine the optimal concentration for your specific assay conditions; typical starting concentrations range from 0.1–1 µg per test.
• Since the immunogen spans amino acids 23–652, the antibody may not detect isoforms lacking this region or those with N-terminal truncations. Validate expression in your target cell system if using alternative isoforms.
• Protect the conjugate from prolonged light exposure to maintain fluorescence stability. Store the antibody as recommended and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material platform, connecting diagnostic manufacturers and researchers with critical reagents like this anti-HER2 monoclonal antibody tailored for flow cytometry applications. Beyond single antibodies, we offer validated antibody pairs, optimized recombinant proteins, and ancillary reagents to support entire oncology and signal transduction assay development. Whether you need bulk supply or assistance in sourcing rare target materials, CamelBio accelerates your journey from concept to clinic.