Rabbit anti-Human HER2/ErbB2 Monoclonal Antibody for FC - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• Receptor tyrosine kinase that is a member of the epidermal growth factor receptor (EGFR/ErbB) family and functions as an essential component of neuregulin-receptor complexes, although neuregulins do not directly bind to it. It requires a coreceptor for ligand binding.
• Upon activation, mediates downstream signaling including the MEMO1-RHOA-DIAPH1 pathway that inhibits GSK3B, leading to stabilization of microtubules at the cell membrane via APC, CLASP2, and MACF1, thereby regulating cell morphology and motility.
• Also localizes to the nucleus where it acts as a transcription factor, binding the PTGS2/COX-2 promoter at the 5'-TCAAATTC-3' sequence to activate transcription, and co-activates CDKN1A expression with STAT3 and SRC. It also enhances rRNA synthesis and protein translation.
• Subcellular localization includes the cell membrane, ruffles, early endosomes, cytoplasm (perinuclear region), and nucleus, reflecting its dynamic signaling and transcriptional roles.
• Post-translational modifications include autophosphorylation at tyrosine residues upon ligand stimulation, and the protein is a glycoprotein with disulfide bonds.
• Overexpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth, and is a well-known oncogene frequently amplified or mutated in cancer.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright green fluorophore, eliminating the need for secondary staining in flow cytometry. Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• The immunogen corresponds to the extracellular domain (amino acids 23-652), suggesting that the antibody likely binds to the cell surface HER2. For FC, use live, unfixed cells to preserve surface staining; if fixation is required, validate that the epitope remains accessible post-fixation.
• Include appropriate isotype controls and compensation controls, especially when combining with other fluorophores. Note that protein weight of HER2 is approximately 138 kDa (predicted) but migrates at ~185 kDa on SDS-PAGE due to glycosylation; this band may be observed in lysates from HER2-overexpressing cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For oncology and targeted therapy research, we offer validated monoclonal antibodies like this anti-HER2/ErbB2 clone, along with optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your diagnostic development pipeline.