Rabbit anti-Human GSK3β Monoclonal Antibody for Flow Cytometry - P49841

Core Product Specifications and Parameters

Biological Background: GSK3β Function and Localization

• GSK3β is a constitutively active serine/threonine-protein kinase that acts as a negative regulator in hormonal control of glucose homeostasis, Wnt signaling, and regulation of transcription factors and microtubules by phosphorylating substrates such as GYS1, CTNNB1, APC, AXIN1, MAPT/TAU, and many others. Related references: PMID:11430833 PMID:12554650 PMID:14690523 PMID:16484495

• In skeletal muscle, GSK3β contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity, linking it to glucose homeostasis and insulin resistance. Related references: PMID:8397507

• In the canonical Wnt signaling pathway, GSK3β forms a multimeric complex with APC, AXIN1, and CTNNB1/beta-catenin, phosphorylating beta-catenin to promote its ubiquitin-proteasome degradation. Related references: PMID:12554650

• GSK3β phosphorylates MAPT/TAU at Thr-548, reducing its microtubule-binding ability; this is relevant to neurofibrillary tangle formation in Alzheimer disease. Related references: PMID:14690523

• It plays a role in circadian rhythm regulation by phosphorylating core clock components BMAL1, CLOCK, and PER2, targeting them for degradation. Related references: PMID:19946213 PMID:28903391

• GSK3β acts as a regulator of autophagy by phosphorylating KAT5/TIP60 under starvation, activating its acetyltransferase function to promote acetylation of ULK1 and RUBCNL. Related references: PMID:30704899

• Subcellular localization includes cytoplasm, nucleus, and cell membrane. Tissue expression is highest in testis, thymus, prostate, and ovary, with weaker expression in lung, brain, and kidney; it colocalizes with PKR and TAU in Alzheimer disease brain.

• GSK3β is subject to multiple post-translational modifications including phosphorylation, ADP-ribosylation, and palmitoylation, which regulate its activity and localization.

Experimental Guidance and Technical Tips

• Given the immunogen design spanning the C-terminal region (aa 321–420), this antibody may recognize both unphosphorylated and phosphorylated GSK3β; validation in the relevant cellular context is recommended.
• For intracellular flow cytometry, optimize permeabilization protocols using methanol or saponin-based buffers to ensure antibody access.
• This antibody has been validated for human GSK3β; cross-reactivity with other species has not been reported.
• The predicted molecular weight of GSK3β is approximately 47 kDa; confirm band size by western blot in your system if applicable.
• As a directly conjugated antibody with , it is ready-to-use for flow cytometry; no secondary antibody is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material sourcing partner for diagnostic manufacturers, laboratories, and research institutes. This GSK3β antibody supports neuroscience, metabolism, and oncology research applications. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, along with specialized sourcing for rare targets to streamline your development pipeline.