Rabbit anti-Human Glypican 3 (GPC3) Monoclonal Antibody for Flow Cytometry - P51654

Core Product Specifications and Parameters

Biological Background: Glypican 3 Function and Localization

• Glypican-3 (GPC3) is a cell surface proteoglycan attached to the membrane via a GPI-anchor, belonging to the glypican family of heparan sulfate proteoglycans. Related references: PMID:14610063
• Negatively regulates the hedgehog signaling pathway by competing with the receptor PTC1 for hedgehog protein binding, leading to internalization and lysosomal degradation of the complex.
• Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and promoting Frizzled-Wnt ligand interaction. Related references: PMID:16227623 PMID:24496449
• Inhibits the dipeptidyl peptidase activity of DPP4, modulating substrate cleavage and downstream cellular processes. Related references: PMID:17549790
• Plays a role in limb patterning, skeletal development, and renal branching morphogenesis by modulating growth factor responses (BMP2, BMP7, FGF7).
• Localized to the cell membrane; detected in placenta, lung, liver, and kidney.
• Contains heparan sulfate chains, a GPI-anchor, and undergoes alternative splicing; classified as a glycoprotein, lipoprotein, and phosphoprotein with disease variant associations.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 481–580, near the C-terminal region of human GPC3; confirm that the antibody recognizes the intended form (full-length or shed) in your system.
• This conjugate is suitable for direct flow cytometry; titrate the antibody to determine the optimal working dilution for your cell type.
• Validate reactivity using GPC3-positive cell lines (e.g., HepG2, A549) or primary tissue samples known to express GPC3.
• Because GPC3 is GPI-anchored, a standard surface staining protocol without permeabilization may be employed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories. In the field of glypican research, we facilitate reliable sourcing of anti-GPC3 products and other rare target reagents, supporting your workflow from early-stage assay development to clinical application.