Rabbit anti-Human GATA3 Monoclonal Antibody for Flow Cytometry (Intracellular) - P23771

Core Product Specifications and Parameters

Biological Background: GATA3 Function and Localization

• GATA3 is a member of the GATA family of zinc-finger transcription factors, also known as GATA-binding factor 3, which recognizes the consensus DNA sequence 5'-AGATAG-3'.
• It functions as a transcriptional activator critical for T-cell development, binding to enhancers of the T-cell receptor alpha and delta genes.
• GATA3 is required for T-helper 2 (Th2) cell differentiation, playing a pivotal role in immune and inflammatory responses.
• Beyond adaptive immunity, GATA3 coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL-33, driving differentiation of alternatively activated macrophages during tissue injury.
• Subcellular localization is predominantly nuclear, consistent with its role as a transcription factor.
• Tissue expression is primarily in T-cells and endothelial cells.
• The protein contains two zinc-finger domains, undergoes phosphorylation, and is subject to alternative splicing, with implications in deafness and other diseases when mutated.

Experimental Guidance and Technical Tips

• As a nuclear target, successful detection by intracellular flow cytometry requires efficient fixation and permeabilization; consider optimizing these steps for your specific cell type.
• Use appropriate T-cell models (e.g., Jurkat cells or primary human T-cells) to confirm specificity, and include isotype controls to set gating thresholds.
• The antibody recognizes an N-terminal epitope (aa 1–200); verify that your experimental conditions do not disrupt this region.
• For multicolor panels, note that is excited at approximately 650 nm and emits at 665 nm; plan compensation accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive platform for sourcing IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For GATA3-related projects, we connect you with high-quality reagents and technical support to streamline research and diagnostic development. Rely on CamelBio as your single partner to accelerate the journey from concept to clinic.