Rabbit anti-Human GATA3 mAb for Flow Cytometry - P23771

Core Product Specifications and Parameters

Biological Background: GATA3 Function and Localization

• GATA3 (GATA-binding factor 3) is a transcription factor belonging to the GATA family, characterized by two conserved zinc-finger domains.
• It binds to the consensus DNA sequence 5'-AGATAG-3' and functions as a transcriptional activator for T-cell receptor alpha and delta genes.
• GATA3 is a master regulator of T-helper 2 (Th2) cell differentiation, critical for immune and inflammatory responses.
• In macrophages, GATA3 coordinates transcriptional activation and UCP2-dependent metabolic reprogramming downstream of IL33 signaling, driving alternative macrophage activation.
• The protein localizes to the nucleus and is primarily expressed in T-cells and endothelial cells.
• Alternative splicing produces multiple isoforms, and the protein undergoes phosphorylation, contributing to its regulatory dynamics.
• Mutations in GATA3 are associated with human disorders such as HDR syndrome (hypoparathyroidism, sensorineural deafness, and renal dysplasia), reflecting its developmental importance.

Experimental Guidance and Technical Tips

• Since the immunogen covers the N-terminal region (aa 1-200) of human GATA3, this antibody may recognize full-length GATA3 and possibly some N-terminal isoforms. Consider verifying isoform specificity in your experimental system.
• The antibody is directly conjugated to , optimized for intracellular flow cytometry (FC intra). Appropriate fixation, permeabilization, and blocking conditions should be optimized for the target cell type.
• For intracellular staining, confirm that the epitope remains accessible after fixation; pilot tests with positive (e.g., Th2-polarized T cells) and negative controls are recommended.
• Given GATA3's role in Th2 differentiation, this antibody can be used to profile Th2 bias in T-cell populations or assess GATA3 expression in macrophage activation studies.
• Titration is recommended to determine the optimal antibody concentration for flow cytometry, balancing specific signal and background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, including validated antibodies for transcription factors like GATA3. We offer bulk quantities, customized conjugations, and ancillary reagents to streamline procurement for diagnostic manufacturers and research laboratories. Our integrated supply chain supports the full development cycle, from target validation to clinical application, with a focus on rare and high-quality raw materials.