Rabbit anti-Human Galectin 3 Monoclonal Antibody for FC (Intra) - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3, also known as L31, GAL3, MAC2, CBP35, GALBP, GALIG, or LGALS2, is a 35 kDa lectin encoded by the LGALS3 gene.
• It functions as a galactose-specific lectin binding IgE and mediating cell-matrix interactions, including stimulation of endothelial cell migration via alpha-3, beta-1 integrin and CSPG4.
• In the nucleus, galectin-3 acts as a pre-mRNA splicing factor, participating in mRNA processing and spliceosome function.
• Galectin-3 is involved in acute inflammatory responses, promoting neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
• Together with TRIM16, it coordinates recognition of endomembrane damage and mobilizes autophagy regulators ATG16L1 and BECN1.
• Subcellular localization includes cytoplasm, nucleus, and secretion.
• Major expression is found in colonic epithelium, activated macrophages, and fetal membranes.
• Post-translational modifications include acetylation and phosphorylation; contains disulfide bonds.

Experimental Guidance and Technical Tips

• The antibody is generated against recombinant human Galectin-3 protein, suggesting recognition of both native and denatured forms.
• For intracellular flow cytometry (FC intra), consider optimizing fixation and permeabilization protocols for your specific cell type.
• Validate specificity using Galectin-3 knockout or knockdown control cells if available.
• Due to confirmed cross-reactivity only with human, verify reactivity in your species of interest if non-human.
• Note that Galectin-3 can be secreted; ensure appropriate cell preparation to avoid target loss.
• The conjugate provides bright fluorescence for sensitive detection; titrate the antibody to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material needs, supporting diagnostic manufacturers from R&D through clinical application. This anti-human Galectin-3 monoclonal antibody is well-suited for immunology and inflammation research, and we can supply validated antibody pairs, optimized recombinant proteins, and ancillary reagents. Our global sourcing network ensures reliable access to rare targets and custom antibody development services.