Rabbit anti-Human Galectin 3 mAb for FC (intra) - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3 is a 35 kDa carbohydrate-binding protein (lectin) also known as 35 kDa lectin, CBP35, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, and Mac-2 antigen.
• Functions as a galactose-specific lectin that binds IgE and mediates, together with alpha-3, beta-1 integrin, stimulation of endothelial cell migration by CSPG4.
• Involved in pre-mRNA splicing in the nucleus, acting as a splicing factor.
• Participates in acute inflammatory responses: neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
• Collaborates with TRIM16 to coordinate recognition of endomembrane damage with mobilization of core autophagy regulators ATG16L1 and BECN1.
• Localizes to the cytoplasm, nucleus, and is secreted; major expression in colonic epithelium and activated macrophages, and found in fetal membranes.
• Post-translational modifications include acetylation, phosphorylation, and disulfide bond formation; implicated in differentiation, innate immunity, and mRNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human Galectin-3 (aa 1-250), producing an antibody that targets the native protein; consider validating in the relevant sample system.
• For intracellular flow cytometry, optimize fixation and permeabilization protocols, as Galectin-3 is expressed in both cytoplasm and nucleus.
• Use an appropriate isotype control and include positive controls such as activated macrophages or colonic epithelial cell lines known to express high levels of Galectin-3.
• Since Galectin-3 is secreted, consider the impact of cell culture conditions on its intracellular detection.
• The antibody is conjugated to , a bright and photostable fluorophore suitable for multiplex flow experiments; ensure proper compensation settings.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between IVD manufacturers and high-quality raw materials, offering a comprehensive portfolio including this anti-Galectin-3 antibody. With expertise in immunology and cancer diagnostics, we provide validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents to streamline your assay development. Whether you require rare target sourcing or optimized raw materials for clinical applications, CamelBio ensures a reliable supply chain from concept to clinic.