Rabbit anti-Human Flt3/CD135 Monoclonal Antibody for Flow Cytometry - P36888

Core Product Specifications and Parameters

Biological Background: Flt3/CD135 Function and Localization

• Encoded by the FLT3 gene, receptor-type tyrosine-protein kinase FLT3 (also known as CD135, FLK-2, FLK2, STK1) is a cell-surface receptor belonging to the class III receptor tyrosine kinase family.
• Acts as a receptor for the cytokine FLT3LG, playing a critical role in the regulation of differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells.
• Upon ligand binding, FLT3 activates downstream signaling pathways including RAS/MAPK, PI3K/AKT/mTOR, and STAT5, through phosphorylation of adaptor proteins and kinases such as SHC1, AKT1, MAPK1/ERK2, MAPK3/ERK1, and STAT5A/B.
• Mutations causing constitutive kinase activity are associated with hematological malignancies, as they promote uncontrolled cell proliferation and resistance to apoptosis.
• Located primarily on the cell membrane as a single-pass transmembrane protein, with its ligand-binding domain exposed extracellularly and kinase domain intracellularly; also detected in the endoplasmic reticulum lumen.
• Highly expressed in bone marrow, hematopoietic stem cells, myeloid progenitor cells, and granulocyte/macrophage progenitor cells; also found in liver, thymus, spleen, lymph node, and at lower levels in kidney and pancreas; notably overexpressed in T-cell leukemia.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, and ubiquitination, which regulate its stability, localization, and activity.
• Alternative splicing generates multiple isoforms, potentially influencing receptor function and tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• This monoclonal antibody is designed for flow cytometry (FC) applications. Perform titration experiments to determine the optimal concentration for your cell type and assay setup.
• The immunogen spans the extracellular domain (aa 27-163), indicating the antibody targets the extracellular region of FLT3. Use appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• As FLT3 is expressed on hematopoietic progenitor and dendritic cells, consider using validated positive (e.g., leukemia cell lines) and negative controls when setting up your flow cytometry panel.
• The direct conjugation eliminates the need for a secondary antibody, simplifying the staining protocol and potentially reducing background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers working on hematopoietic malignancies or stem cell biology, we provide reliable access to high-quality anti-FLT3 antibodies like this conjugate. Our one-stop service bridges the gap from concept to clinic, supporting your assay development with technical consultation and supply chain efficiency.