Rabbit anti-Human FGFR3 Monoclonal Antibody for Flow Cytometry - P22607

Core Product Specifications and Parameters

Biological Background: FGFR3 Function and Localization

• FGFR3 (Fibroblast growth factor receptor 3) is a receptor tyrosine kinase that binds fibroblast growth factors, regulating cell proliferation, differentiation, and apoptosis.
• Essential for chondrocyte differentiation and skeletal development; required for normal osteogenesis and postnatal bone mineralization.
• Signals through multiple pathways: phosphorylates PLCG1 (producing DAG and IP3) and FRS2, leading to activation of RAS-MAPK and AKT1 pathways.
• Plays a role in vitamin D metabolism and inner ear development; a secreted isoform (isoform 3) may interfere with FGF signaling by binding ligands.
• Expressed in brain, kidney, testis, and fetal tissues; isoform-specific expression in epithelial versus fibroblastic cells.
• Subcellular locations include cell membrane, cytoplasmic vesicles, endoplasmic reticulum, and secreted forms.
• Associated with numerous disease variants: mutations linked to craniosynostosis, deafness, dwarfism, ectodermal dysplasia, and lacrimo-auriculo-dento-digital syndrome.
• Post-translational modifications: glycoprotein, phosphorylation, and Ubl conjugation; contains immunoglobulin domains and a kinase domain.

Experimental Guidance and Technical Tips

• As a recombinant protein-derived rabbit monoclonal antibody for flow cytometry (FC), consider performing an antibody titration to determine the optimal concentration for your cell type.
• The predicted molecular weight of FGFR3 is 88kDa; verify target expression in your sample using appropriate positive and negative controls.
• FGFR3 exhibits tissue-specific expression; for FC applications, select cell lines or primary cells known to express FGFR3 (e.g., brain, kidney, testis-derived cells) and confirm with isotype controls.
• If studying secreted isoform 3, validate that the antibody recognizes the relevant epitope, as the immunogen is a recombinant protein that may not cover all isoforms.
• is a bright fluorophore; ensure your flow cytometry panel design accounts for potential spectral overlap, especially with other far-red dyes.
• Shipping on ice bag; upon receipt, store according to the manufacturer's recommendations and avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, offering validated monoclonal antibodies, bulk reagents, and technical support. This FGFR3 antibody supports flow cytometry applications in oncology and skeletal development research, with reliable supply chains for diagnostic development. From rare target sourcing to ancillary reagents, CamelBio bridges the gap from concept to clinic.