Rabbit anti-Human FATP1 Monoclonal Antibody for Intracellular Flow Cytometry - Q6PCB7

Core Product Specifications and Parameters

Biological Background: FATP1 Function and Localization

• FATP1 (SLC27A1) is a long-chain fatty acid transport protein 1, also known as fatty acid transport protein 1, ACSVL5, or arachidonate--CoA ligase.
• It mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating transport at the plasma membrane, as supported by functional studies (PubMed:12556534, PubMed:20530735, PubMed:21395585). Related references: PMID:12556534 PMID:20530735 PMID:21395585
• FATP1 also functions as an acyl-CoA ligase, catalyzing ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA), which prevents efflux and promotes intracellular accumulation.
• Subcellularly, FATP1 localizes to the cell membrane, endomembrane system, and cytoplasm, consistent with its dual transporter/ligase roles.
• Tissue specificity: highest expression in muscle and adipose tissue, intermediate in small intestine, and barely detectable in liver; also expressed in brain gray matter (PubMed:10873384, PubMed:21395585). Related references: PMID:10873384 PMID:21395585
• The protein plays a pivotal role in regulating exogenous LCFA substrates in tissues undergoing high levels of beta-oxidation or triglyceride synthesis.
• UniProt keywords highlight its involvement in fatty acid metabolism, lipid transport, and alternative splicing, with a calculated molecular weight of ~71 kDa (71,108 Da). Observed bands at 52 kDa and 71 kDa suggest potential isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 510-646 of human FATP1; when using this conjugate for intracellular flow cytometry, optimize permeabilization protocols to ensure antibody access to cytoplasmic epitopes.
• Because the antibody is a pre-conjugated monoclonal, direct staining eliminates the need for secondary antibodies but requires careful titration to achieve optimal signal-to-noise ratios.
• The observed molecular weights (52 kDa and 71 kDa) may reflect alternative splicing or post-translational modifications; consider these when interpreting western blot validation (if performed separately).
• As FATP1 is expressed at different levels across tissues, choose appropriate positive controls (e.g., adipose or muscle-derived cells) and isotype controls for FC gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research labs with validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need ready-to-use conjugates for FATP1-related metabolic studies or custom rare-target raw-material procurement, CamelBio bridges the gap from concept to clinic. Our integrated supply chain ensures streamlined access to high-quality materials for fatty acid transport research.