Rabbit anti-Human ERG Monoclonal Antibody for Intracellular Flow Cytometry - P11308

Core Product Specifications and Parameters

Biological Background: ERG Function and Localization

• ERG (ETS-related gene) is a transcriptional regulator encoded by the ERG gene, also known as transforming protein ERG or p55.
• It is a DNA-binding transcription factor belonging to the ETS family, playing a role in gene expression regulation.
• ERG may modulate chromatin structure by recruiting the SETDB1 histone methyltransferase to target genes.
• Subcellular localization includes both the nucleus and cytoplasm.
• Post-translational modifications such as phosphorylation and ubiquitination (isopeptide bond formation) are reported for ERG.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• ERG is characterized as a proto-oncogene, frequently rearranged in cancers like prostate adenocarcinoma and Ewing's sarcoma.
• The canonical ERG protein has a molecular weight of approximately 54 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human ERG, suggesting recognition of a linear epitope. This may be useful for detecting denatured ERG in intracellular staining.
• For intracellular flow cytometry, optimize cell fixation and permeabilization conditions (e.g., using paraformaldehyde fixation followed by methanol or detergent permeabilization) to ensure antibody access to nuclear ERG.
• Since the antibody is directly conjugated to , no secondary staining step is required, reducing assay time and potential background.
• Validate the staining specificity by including appropriate isotype control antibodies and testing ERG-positive vs. negative cell lines (e.g., VCaP, LNCaP as positive; ERG-negative lines as negative).
• The predicted molecular weight of ERG is 54 kDa; if additional confirmation is needed, consider parallel analysis by western blot using unconjugated anti-ERG antibodies.

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CamelBio is your one-stop partner for IVD raw material sourcing, providing high-quality antibodies like this anti-ERG monoclonal antibody for cancer and transcription research. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. From concept to clinic, we support your diagnostic development with reliable raw materials and expert technical services.