Rabbit anti-Human ERG Monoclonal Antibody for Flow Cytometry (Intracellular) - P11308

Core Product Specifications and Parameters

Biological Background: Transcriptional regulator ERG Function and Localization

• ERG (ETS-related gene) is a transcriptional regulator, also known as transforming protein ERG, p55, or erg-3, encoded by the ERG gene.
• It functions as a DNA-binding transcription factor that may regulate gene expression by recruiting SETDB1 histone methyltransferase, thereby modifying local chromatin structure.
• ERG is a member of the ETS family of transcription factors, characterized by a conserved ETS DNA-binding domain.
• Subcellular localization includes both the nucleus and cytoplasm, consistent with its role in transcriptional regulation and signaling.
• The protein undergoes alternative splicing and is subject to post-translational modifications such as phosphorylation, isopeptide bond formation, and ubiquitination.
• ERG is implicated in oncogenic chromosomal rearrangements, notably in prostate cancer where TMPRSS2-ERG gene fusions lead to overexpression.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry (FC intra), use appropriate fixation and permeabilization buffers to ensure antibody access to nuclear and cytoplasmic ERG.
• Since the immunogen is a peptide from the N-terminal region (aa 1-100) of isoform NP_891548.1, verify that your target cell line expresses this specific isoform.
• Consider titrating the antibody to determine the optimal concentration for your experimental system; avoid over-staining.
• Cross-reactivity is confirmed for human; if using with other species, validate specificity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting diagnostic developers from concept to clinic. This anti-ERG antibody serves research in transcriptional regulation and oncology, particularly ERG-related cancers. We provide validated monoclonal and polyclonal antibodies, bulk reagents, and sourcing of rare targets to streamline your assay development.