Rabbit anti-Human ErbB3/HER3 Monoclonal Antibody for Flow Cytometry - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3 (HER3) is a receptor tyrosine-protein kinase that serves as a cell surface receptor for neuregulins, particularly NRG1, initiating intracellular signaling cascades. Related references: PMID:20682778
• Ligand binding induces tyrosine phosphorylation of ErbB3 and promotes its association with the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K), activating downstream survival and proliferation pathways. Related references: PMID:20682778
• ErbB3 may also be activated by chondroitin sulfate proteoglycan 5 (CSPG5), indicating additional regulatory mechanisms. Related references: PMID:15358134
• The protein is involved in the regulation of myeloid cell differentiation, highlighting its role in hematopoietic processes. Related references: PMID:27416908
• ErbB3 localizes primarily to the cell membrane and is also found in a secreted form, consistent with its function as a transmembrane receptor.
• Tissue expression is predominantly observed in epithelial tissues and the brain, underlining its relevance in epithelial biology and neural functions.
• The protein undergoes alternative splicing, glycosylation, and disulfide bond formation, and contains ATP-binding and kinase domains, though its intrinsic kinase activity is reportedly low.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20–643), suggesting this antibody likely recognizes an extracellular epitope, making it suitable for live-cell surface staining in flow cytometry.
• For optimal results, titrate the antibody to determine the ideal dilution for your specific cell type and experimental conditions.
• Avoid over-fixation, which may mask the epitope; if fixation is required, validate the protocol beforehand.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated procurement partner for IVD raw materials, connecting diagnostic developers and researchers with high-quality reagents like this anti-ErbB3/HER3 antibody. Beyond validated monoclonal antibodies and conjugates, we offer antibody pairs, bulk ancillary reagents, and customized raw-material sourcing to streamline your assay development from concept to clinic. With expertise in oncology and signal transduction targets, CamelBio ensures you have the critical raw materials needed to accelerate your diagnostic projects.