Rabbit anti-Human ErbB3/HER3 Monoclonal Antibody () for Flow Cytometry - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3 (HER3) is a receptor tyrosine-protein kinase that serves as a cell surface receptor for neuregulins, primarily NRG1. Ligand binding triggers tyrosine phosphorylation and promotes association with the p85 subunit of phosphatidylinositol 3-kinase, activating downstream signaling. Related references: PMID:20682778
• ErbB3 can also be activated by CSPG5, expanding its role in neural contexts. Related references: PMID:15358134
• ErbB3 is involved in the regulation of myeloid cell differentiation, linking it to hematopoietic processes. Related references: PMID:27416908
• ErbB3 is predominantly expressed in epithelial tissues and brain, consistent with its role in development and cancer.
• Subcellularly, ErbB3 is located primarily at the cell membrane but a secreted form also exists, as indicated by alternative splicing.
• ErbB3 undergoes multiple post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation, which are critical for its structure and function.
• Alternative splicing generates several isoforms with predicted molecular weights ranging from 20 kDa to 148 kDa, as reflected in the observed protein weight pattern.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a bright and photostable fluorophore, and is supplied ready-to-use for flow cytometry. It targets an epitope within the extracellular domain (amino acids 20–643), making it suitable for surface staining of live, unfixed cells.
• For flow cytometry, consider titrating the antibody to determine the optimal dilution for your specific cell type and experimental conditions. Include appropriate isotype controls and fluorescence minus one (FMO) controls to set gates accurately.
• Since cross-reactivity is reported only for human, we recommend validating performance in human-derived samples. If using with other species, sequence alignment of the immunogen region is advised.
• The antibody may also be compatible with immunofluorescence and other surface-detection methods, but such applications should be empirically validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes by providing end-to-end access to IVD raw materials, technical services, and consulting—from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing tailored to your assay development needs. Whether you are building an ErbB3/HER3-related diagnostic kit or exploring neuregulin signaling pathways, CamelBio serves as your reliable sourcing partner, streamlining procurement so you can focus on innovation.