Rabbit anti-Human ErbB3/HER3 Monoclonal Antibody for Flow Cytometry - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3 is a receptor tyrosine-protein kinase that functions as a cell surface receptor for neuregulins, primarily neuregulin-1 (NRG1). Ligand binding induces tyrosine phosphorylation and promotes association with the p85 subunit of phosphatidylinositol 3-kinase (PI3K). It may also be activated by CSPG5. Related references: PMID:20682778 PMID:15358134
• ErbB3 is involved in the regulation of myeloid cell differentiation, underscoring its role in hematopoietic lineage commitment. Related references: PMID:27416908
• The ERBB3 gene undergoes alternative splicing, generating multiple isoforms, including secreted variants that lack the transmembrane domain. This is consistent with its annotation as a secreted protein in addition to its membrane-bound form.
• Subcellularly, ErbB3 is predominantly localized to the cell membrane, with secreted isoforms found extracellularly. Tissue expression is notable in epithelial tissues and the brain.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation on key tyrosine residues, which are critical for its signaling functions.
• As a member of the ERBB family of receptor tyrosine kinases, ErbB3 contains an ATP-binding domain and exhibits tyrosine kinase activity, though its kinase activity is reportedly weaker than other family members.
• Disease-associated variants of ERBB3 have been linked to conditions such as lethal congenital contracture syndrome 2 (LCCS2) and other disorders, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the large extracellular domain (aa20-643) of human ErbB3, suggesting the antibody is likely to recognize the native cell surface receptor. For flow cytometry, consider using live, unfixed cells to maintain surface epitope integrity.
• Titrate the antibody to determine the optimal concentration for your specific application and include appropriate isotype controls and fluorescence-minus-one (FMO) controls.
• ErbB3 expression is reportedly prominent in epithelial tissues and certain brain regions. Validate in relevant cell lines (e.g., MCF-7, T47D) or primary samples known to express the target.

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