Rabbit anti-Human EGFR Monoclonal Antibody for IF/ICC, FC - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR, also known as ERBB1/HER1) is a transmembrane receptor tyrosine kinase that specifically binds EGF family ligands (e.g., EGF, TGF-α, AREG, HB-EGF). Ligand engagement induces receptor dimerization and autophosphorylation, initiating intracellular signal transduction.
  Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029
• Activated EGFR triggers multiple downstream signaling modules, including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STATs, controlling cell proliferation, survival, and differentiation.
  Related references: PMID:27153536
• EGFR also directly phosphorylates RGS16 (modulating G-protein signaling), MUC1 (promoting its interaction with SRC and β-catenin), and interacts with CCDC88A/GIV to promote cell migration.
  Related references: PMID:11602604 PMID:11483589 PMID:20462955
• Isoform 2 of EGFR, generated by alternative splicing, may act as an antagonist of EGF action, counterbalancing the canonical signaling.
• EGFR localizes primarily at the cell membrane but undergoes ligand-induced endocytosis and can be found in endosomes, the nucleus, endoplasmic reticulum, and Golgi apparatus. This dynamic trafficking regulates signal duration and specificity.
• Post-translational modifications, including phosphorylation, ubiquitination, glycosylation, and palmitoylation, fine-tune EGFR activity, stability, and compartmentalization.
• Ubiquitously expressed in normal tissues, EGFR is frequently overexpressed or mutated in cancers; isoform 2 is notably expressed in ovarian cancers.
• EGFR serves as a host entry factor for hepatitis C virus (HCV) in hepatocytes by facilitating the formation of CD81-CLDN1 co-receptor complexes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 335–525 of human EGFR, a region within the extracellular ligand-binding domain. This design may confer specificity for the receptor's native conformation in cell-surface staining applications.
• For immunofluorescence and flow cytometry, optimal working dilutions should be empirically determined for each cell line or tissue type. Consider using appropriate fixation/permeabilization protocols to preserve antigenicity.
• This fluorophore-conjugated antibody eliminates the need for secondary detection, reducing non-specific background and protocol time. Validate reactivity in the intended human sample to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, connecting you with high-quality antibodies, bulk ancillary reagents, and expert technical support. For EGFR-related research and diagnostic development, our portfolio includes validated antibody pairs and optimized monoclonal antibodies like this conjugate, ensuring reliable performance. Leverage our rare target material sourcing and consulting services to accelerate your assay development from concept to clinic.