Rabbit anti-Human EGFR Monoclonal Antibody for IF/ICC and FC - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that binds EGF family ligands and activates downstream signaling cascades, including the RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways, to regulate cellular responses such as proliferation and survival.
  Related references: PMID:10805725, PMID:27153536, PMID:2790960
• Known EGFR ligands include EGF, TGF-α, amphiregulin (AREG), epigen (EPGN), betacellulin (BTC), epiregulin (EREG), and heparin-binding EGF (HBEGF).
  Related references: PMID:12297049, PMID:15611079, PMID:17909029
• EGFR may also activate the NF-kappa-B signaling pathway.
  Related references: PMID:11116146
• The receptor directly phosphorylates other proteins, such as RGS16 (linking to G protein signaling) and MUC1 (promoting interaction with SRC and β-catenin).
  Related references: PMID:11602604, PMID:11483589
• EGFR positively regulates cell migration through its interaction with CCDC88A/GIV, which retains the receptor at the cell membrane following ligand stimulation.
  Related references: PMID:20462955
• Isoform 2 of EGFR may function as an antagonist of EGF action and is notably expressed in ovarian cancers.
• Subcellular locations include the cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane, nucleus membrane, endosome, nucleus, and secreted forms. The protein undergoes extensive post-translational modifications, including glycosylation, phosphorylation, ubiquitination, and palmitoylation.
• EGFR also serves as a host receptor for hepatitis C virus (HCV) entry, mediating the formation of CD81-CLDN1 complexes and enhancing viral membrane fusion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 335–525) of human EGFR. As the antibody is directly conjugated to , it is immediately applicable for immunofluorescence (IF/ICC) and flow cytometry (FC) without the need for secondary detection.
• For IF/ICC, consider appropriate fixation and permeabilization protocols to preserve the protein’s native conformation and epitope accessibility in your specific cell type.
• The antibody is specific to human EGFR; verify cross-reactivity if modeling other species.
• Include positive and negative controls (e.g., EGFR-overexpressing vs. knockout cell lines) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive source for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinical application. This anti-EGFR mAb is an example of our readily available conjugated antibody solutions for cell biology and oncology research. Should your project require custom antibody sourcing, rare-target reagent development, or technical consulting, our integrated supply chain and expertise ensure efficient and reliable procurement.